PICK1在肝细胞癌中的表达及其临床意义  

Expression of PICK1 in hepatocellular carcinoma and its clinical significance

作　　者：程文华[1] 柳婧美[1] 杨文慧[1] Cheng Wenhua;Liu Jingmei;Yang Wenhui(Department of Gastroenterology,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院、中国医学科学院肿瘤医院山西医院、山西医科大学附属肿瘤医院消化内科,太原030013

出　　处：《肿瘤研究与临床》2024年第11期824-828,共5页Cancer Research and Clinic

基　　金：山西省肿瘤医院国家区域医疗中心科教培育基金(QH2023027)。

摘　　要：目的探讨PICK1在肝细胞癌(HCC)中的表达及其临床意义。方法回顾性病例系列研究。选择2020年8月至2023年4月于山西省肿瘤医院行手术切除30例HCC患者的新鲜肿瘤组织和癌旁组织(距离癌组织边缘1 cm), 采用实时荧光定量聚合酶链反应(qRT-PCR)检测PICK1 mRNA的相对表达量。同时收集2015年1月至2018年11月于山西省肿瘤医院行手术切除的132例HCC患者的临床病理资料, 以及癌组织和癌旁组织的石蜡标本, 应用免疫组织化学法检测PICK1蛋白的相对表达量。根据132例患者PICK1蛋白的表达情况, 免疫组织化学评分<3分为低表达组, ≥3分为高表达组;采用Kaplan-Meier法比较两组总生存(OS)、无病生存(DFS), 并进行log-rank检验。结果 qRT-PCR检测结果显示, 30例HCC患者的癌组织中PICK1 mRNA的相对表达量低于相应的癌旁组织, 差异有统计学意义(0.43±0.38比1.10±0.12, t=-13.66, P<0.001)。132例HCC患者中, PICK1蛋白低表达86例(65.2%), 高表达46例(34.8%);相应的癌旁组织中, PICK1蛋白低表达37例(28.0%), 高表达95例(72.0%), HCC组织中PICK1蛋白高表达率低于癌旁组织, 差异有统计学意义(χ^(2)=35.07, P<0.001)。不同肿瘤长径、肿瘤数目、肿瘤类型、TNM分期及是否有包膜形成、血管侵犯的HCC患者PICK1蛋白表达情况比较, 差异均有统计学意义(均P<0.05)。Kaplan-Meier生存分析结果显示, PICK1高表达组术后中位OS时间为54.9个月(95%CI:41.0个月至未达到), 低表达组为17.8个月(95%CI:13.1~25.0个月), PICK1高表达组OS优于低表达组, 差异有统计学意义(P<0.001)。PICK1高表达组中位DFS时间为43.0个月(95%CI:31.0个月至未达到), 低表达组为15.0个月(95%CI:11.1~20.1个月), PICK1高表达组DFS优于低表达组, 差异有统计学意义(P<0.001)。结论 PICK1在HCC组织中低表达, PICK1低表达患者预后较差。Objective:To explore the expression of PICK1 in hepatocellular carcinoma(HCC)and its clinical significance.Methods:A retrospective case series study was conducted.Cancer tissues and paracancerous tissues(1cm from the edge of cancer tissues)were collected from 30 HCC patients undergoing surgical resection in Shanxi Province Cancer Hospital from August 2020 to April 2023.The relative expression level of PICK1 mRNA in 30 fresh HCC samples was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The clinicopathological data from 132 HCC patients undergoing surgical resection in Shanxi Province Cancer Hospital from January 2015 to November 2018 were collected.Meanwhile,paraffin specimens were collected from cancer tissues and paracancerous tissues,and relative expression level of PICK1 protein was detected by using immunohistochemistry method.According to the PICK1 protein expression,132 HCC patients were divided into the low expression group(immunohistochemistry scores<3 points)and the high expression group(immunohistochemistry scores≥3 points).Kaplan-Meier method was used to compare the overall survival(OS)and disease-free survival(DFS)of the 2 groups,and log-rank test was also performed.Results:The result of qRT-PCR showed that the relative expression level of PICK1 mRNA in fresh cancer tissues of 30 HCC patients was lower than that in corresponding paracancerous tissues(0.43±0.38 vs.1.10±0.12),and the difference was statistically significant(t=-13.66,P<0.001).Among 132 HCC patients,86(65.2%)cases had PICK1 protein low expression and 46(34.8%)cases had PICK1 protein high expression;among the corresponding paracancerous tissues,37(28.0%)tissues had PICK1 protein low expression and 95(72.0%)tissues had PICK1 protein low expression.The high expression rate of PICK1 protein in HCC tissues was lower than that in paracancerous tissues,and the difference was statistically significant(χ^(2)=35.07,P<0.001).There were statistically significant differences in PICK1 protein expression level of HCC patient

关 键 词：癌 肝细胞 基因 PICK1 预后 

分 类 号：R735.7[医药卫生—肿瘤]