响应面法优化灵芝多糖提取工艺及其抗氧化活性  

Optimization of Extraction Technology and Antioxidant Activity of Ganoderma lingzhi by Response Surface Method

作　　者：颜瑞辰 洪允炬 李悦 李长田 陈春宇 张影 华霜 YAN Ruichen;HONG Yunju;LI Yue;LI Changtian;CHEN Chunyu;ZHANG Ying;HUA Shuang(College of Traditional Chinese Medicine,Jilin Agricultural Science and Technology University,Jilin 132109,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;Engineering Research Center of Edible and Medicinal Fungi,Ministry of Education,Changchun 130118,China;College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;School of Pharmacy of Chongqing Three Gorges Medical College,Chongqing 404120,China)

机构地区：[1]吉林农业科技学院中药学院,吉林132109 [2]中国科学院天津工业生物技术研究所,天津300308 [3]食药用菌教育部工程研究中心,长春130118 [4]吉林农业大学植物保护学院,长春130118 [5]重庆三峡医药高等专科学校药学院,重庆404120

出　　处：《菌物研究》2024年第4期378-386,共9页Journal of Fungal Research

基　　金：吉林省教育厅项目(JJKH20220395KJ);吉林农业科技学院博士人才基金项目(111022022020);三峡库区道地药材开发利用重庆市重点实验室项目(Sys20210014);天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006)。

摘　　要：为提高灵芝Ganoderma lingzhi多糖的提取率,利用响应面分析法优化灵芝多糖提取工艺。分别考察灵芝多糖提取方法、提取时间、料液比、提取次数对多糖得率的影响。结果表明,采用回流提取方式多糖得率最高,该方式下最佳条件为提取温度85℃,提取时间4.2 h,料液比例1∶31,提取2次,提取率为1.676%。灵芝子实体多糖体外抗氧化活性结果表明,灵芝子实体多糖在1 mg/mL质量浓度下对1,1-二苯基-2-三硝基苯肼(DPPH)自由基与羟自由基清除率分别为94.23%和61.21%。研究结果为灵芝多糖的提取及资源开发利用提供参考。Response surface analysis was used to optimize the extraction process of Ganoderma lingzhi polysaccharides for the improvement of extraction rates and study of their antioxidant activity.Effects of methods,used time,solid-liquid ratio and number of times for extraction on polysaccharide yields were investigated.The results showed that reflux extraction method had the best extraction rate under the mostly suitable conditions as indicated below:temperature 85℃,time 4.2 h,solid-liquid ratio 1∶31 and twice extractions,which revealed the extraction rate of 1.676%.At the concentration of 1 mg/mL of G.lingzhi fruit body polysaccharides,the scavenging rates of DPPH free radical and hydroxyl-free radical were 94.23%and 61.21%,respectively.Our study thereafter provides a basic reference for the effective extraction of G.lingzhi polysaccharides and the development and utilization of their resources in future.

关 键 词：灵芝 回流提取法 响应面法 多糖 抗氧化活性 

分 类 号：Q949.32[生物学—植物学] R284[医药卫生—中药学]