宁夏鼠伤寒沙门菌及其单相变种基因组特征分析  

Genomic characterization of Salmonella typhimurium and its monophasic variants in Ningxia

作　　者：刘翔[1] 张燕飞[1] 魏琼[1] 杨丽娟 李娜[1] 马学旻[1] 郭邦成[1] LIU Xiang;ZHANG Yan-fei;WEI Qiong;YANG Li-juan;LI Na;MA Xue-min;GUO Bang-cheng(Ningxia Center for Disease Control and Prevention,Yinchuan 750002,China)

机构地区：[1]宁夏回族自治区疾病预防控制中心,银川750002

出　　处：《中国人兽共患病学报》2024年第11期1018-1023,1035,共7页Chinese Journal of Zoonoses

基　　金：国家科技重大专项(No.2018ZX10713-003);宁夏自然科学基金(No.2022AAC03711)资助。

摘　　要：目的从全基因组水平上探讨宁夏地区鼠伤寒沙门菌(Salmonella typhimurium,S.typhimurium)及其单相变种(简称S.1,4,[5],12:i:-)流行特征,建立全基因分型数据库,为今后的暴发溯源及防控提供理论依据。方法采用全基因组测序技术对宁夏地区分离的92株鼠伤寒沙门菌及其单相变种进行血清型分型、MLST、cgMLST分型、毒力基因及耐药表型预测。结果所有菌株中,21株为S.typhimurium,占22.83%;71株为S.1,4,[5],12:i:-,占77.17%。MLST和cgMLST分型结果显示,S.typhimurium均为ST19型,分为5个进化分支,发现5个聚类簇;S.1,4,[5],12:i:-均为ST34型,同在一个进化分支上,发现17个聚类簇。VFDB预测共发现8类155种毒力基因,其中33.33%的S.typhimurium中携带质粒上的9种毒力基因,2种血清型均携带染色体上与SPI毒力岛相关毒力基因。耐药表型预测结果显示,所有S.1,4,[5],12:i:-耐4~14种抗生素,产生22种多重耐药谱,S.typhimurium耐2~13种抗生素,产生8种多重耐药谱。结论宁夏地区S.1,4,[5],12:i:-为继肠炎沙门菌的主要流行优势血清型,其耐药性和遗传多样性高于S.typhimurium,而携带毒力基因数量低于S.typhimurium,且均存在较高的局部暴发风险。This study was aimed at exploring the epidemiological characteristics of Salmonella typhimurium(S.typhimurium)and Salmonella typhimurium monophasic variants(S.1,4,[5],12:i:-)at the genome-wide level in Ningxia.Establishment of a whole genome genotyping database would provide a theoretical basis for source and prevention and control of future outbreaks.We conducted serotyping,MLST,cgMLST typing,and virulence gene and drug resistance phenotype prediction of 92 strains of S.typhimurium and its monophasic variants through whole genome sequencing.Among all strains,21 were S.typhomurium,accounting for 22.83%,71 strains were S.1,4,[5],12:i:-,accounting for 77.17%.MLST and cgMLST typing results indicated that all S.typhimurium strains were ST19-type,and were divided into five evolutionary branches.Five clusters were found,and S.1,4,[5],12:i:-were all ST34-type;on the same evolutionary branch,17 clusters were found.VFDB predicted 155 virulence genes in eight classes,of which 33.33%were S.typhimurium carried nine virulence genes on plasmids,and both serotypes carried virulence genes related to SPI virulence island on chromosomes,12:I:-resistant to 4-14 antibiotics,resulting in 22 multi-drug resistance spectrum.S.typhimurium was resistant to 2-13 antibiotics and produced eight multi-drug resistance profiles.S.1,4,[5],12:i:-was the predominant serotype of S.enteritidis in Ningxia.Typhimurium carried fewer virulence genes than S.typhimurium,and showed a high risk of local outbreaks.

关 键 词：鼠伤寒沙门菌 全基因组测序 毒力基因 耐药表型 

分 类 号：R378.2[医药卫生—病原生物学]