Using transcriptomics to reveal the molecular mechanism of higher alcohol metabolism in Saccharomyces cerevisiae  

作　　者：Danyao Cui Ling Liu Xiaoyuan Zhang Liangcai Lin Xin Li Ting Cheng Chunhui Wei Yu Zhang Zheng Zhou Wei Li Cuiying Zhang 

机构地区：[1]Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin,300457,PR China [2]Shandong Acad Pharmaceut Sci,Natl Local Joint Engn Lab Polysaccharide Drugs,Key Lab Biopharmaceut Engn Lab Polysaccharid,Jinan,250101,PR China [3]Liquor Making Biological Technology and Application of Key Laboratory of Sichuan Province,Sichuan,643002,PR China

出　　处：《Food Bioscience》2023年第1期210-219,共10页食品生物科学（英文）

基　　金：Supported by Tianjin Science and Technology Project(22ZYJDSS00050);Project Program of Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,and Tianjin Key Laboratory of Industrial Microbiology,China(No.2020KF002);National Natural Science Foundation of China(31771969);Liquor Making Biological Technology and Application of key laboratory of Sichuan Province(NJ2020-02);Project Program of Tianjin Graduate Research and Innovation(No.2020YJSB124).

摘　　要：High alcohols are important flavor compounds in alcoholic beverages,but high concentration of high alcohols is harmful to human health.Higher alcohol synthesis pathways in brewing microorganisms have been investigated,but the interactions between key genes remain to be explored,especially in industrial strains of Saccharomyces cerevisiae.The PDC1 gene was considered to be the main encoding gene ofα-keto acid decarboxylase,and its deletion resulted in a 92.23%increase in isobutanol production and a 14.89%decrease in isoamyl alcohol production.Transcriptome sequencing was used to explore the effects of PDC1 gene deletion on global gene transcription levels,and deletion strategies were utilized to verify the effects of differential genes on higher alcohol production.Deletion of differential gene HMLALPHA2 increased isobutanol production by 26.23%,and decreased isoamyl alcohol and 2-phenylethanol production by 30.31%and 22.35%,respectively.The THI2,THI4,THI20 genes were proved to be related to n-propanol synthesis.In addition,HMRA2 and SIR3 genes were found to influence isoamyl alcohol synthesis pathways,and their deletion increased isoamyl alcohol production by 25.18%and 21.76%.Our discovery of new target genes is useful for elucidating the molecular mechanisms of higher alcohols and the construction of novel low-producing higher alcohol strains.

关 键 词：Saccharomyces cerevisiae Higher alcohol Transcriptomics sequencing Alcoholic beverage α-keto acid decarboxylase PDC1 gene 

分 类 号：TQ923[轻工技术与工程—发酵工程]