Melanin from Sepia pharaonis ink alleviates mucosal damage and reduces inflammation to prevent alcohol-induced gastric ulcers  

作　　者：Lin Liu Kuan Lu Jingwen Xie Hongxia Che Hongyan Li Xie Wancui 

机构地区：[1]College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Shandong,Qingdao,266042,China [2]College of Food Science and Engineering,Qingdao Agricultural University,Shandong,Qingdao,266109,China [3]Qingdao Special Food Research Institute,Shandong,Qingdao,266109,China [4]Shandong Provincial Key Laboratory of Biochemical Engineering,Shandong,Qingdao,266042,China [5]Qingdao Keda Future Biotechnology Co.,Ltd.,Shandong,Qingdao,266042,China

出　　处：《Food Bioscience》2023年第1期508-519,共12页食品生物科学（英文）

基　　金：The National Key R&D Program of China has funded this work(2018YFD0901105).

摘　　要：Melanin(MSI)is the primary component of Sepia pharaonis ink.Studies have demonstrated melanin’s antiinflammatory and antioxidant activities in colitis.In the present study,the protective effect of ink melanin against ethanol-induced gastric mucosal damage in mice was investigated.After oral administration of MSI for 12 days,the mice were treated with anhydrous ethanol(0.2 mL)to establish the gastric ulcer model.Histopathological analysis showed that MSI reduced the ulcer area and bleeding symptoms of gastric tissue.MSI exhibited significant antioxidant properties by upregulating superoxide dismutase(SOD),glutathione(GSH),and catalase(CAT)activities and decreasing malondialdehyde(MDA)content.The expression levels of epidermal growth factor(EGF),epidermal growth factor receptor(EGFR),and prostaglandin E-2(PGE2)significantly decreased during gastric mucosal injury,while MSI significantly reversed the effects.Furthermore,MSI reduced the expression of inflammatory factors tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β)and the protein expression of toll-like receptor 4(TLR4),p38 mitogen-activated protein kinase(p38 MAPK)and extracellular signal-regulated protein kinases 1/2(ERK1/2).In addition,MSI repressed gastric cell apoptosis by decreasing the expression of Caspase-3.In conclusion,MSI alleviated ethanol-induced gastric ulcer injury by modulating oxidation stress and inflammatory cytokines and maintaining the mucosal barrier,which can be used as therapeutic functional food or nutritional supplement to prevent gastric ulcers.Meanwhile,the mechanism of MSI targeted gastric TLR4/MAPKs pathway deserves further verification.

关 键 词：Ethanol Gastric mucosal damage ANTI-INFLAMMATORY Ink melanin 

分 类 号：R573.1[医药卫生—消化系统]