The cytoprotection of milk-derived MFG-E8 on mitochondria-injured L6 cell via mediation of Akt/bcl-2/bax-caspase-3 signaling pathway  

作　　者：Kaifang Guan Xiaofen Qi Haoran Chen Ying Ma 

机构地区：[1]School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin,150090,Heilongjiang,PR China

出　　处：《Food Bioscience》2023年第1期625-634,共10页食品生物科学（英文）

摘　　要：Mitochondria-dependent myoblast apoptosis induced by oxidative stress and decrease in successful protein synthesis play a crucial role in loss of muscle mass and functionality further to result in sarcopenia.Here,we investigated the relationship between milk fat globule-EGF factor VIII(MFG-E8)and mitochondrial-dependent apoptosis pathway.MFG-E8 exert cytoprotection through increasing L6 cells survival/apoptotic ratio,increasing mitochondrial membrane potential and regulating S and G2/M phases.By observing cell ultrastructure,MFG-E8 improved mitochondrial homeostasis mainly through decreasing cytochrome-c release,expression of caspase-9 and caspase-3,mitochondrial vacuolation and mitophagy thereby inhibiting cell apoptosis.From molecular perspective,MFG-E8 repaired mitochondria fragmentation by increasing mitochondrial DNA replication and regulated expression of key mitochondrial-apoptotic factors(upregulation:B-cell lymphoma-2 like 1(bcl2l1),bcl2l2,cyclooxygenase-1 and mitochondrial uncoupling protein-2;Downregulation:p21 and p53)further to improve mitochondrial function and inhibit apoptosis.Moreover,MFG-E8 inhibited mitochondrialdependent apoptosis via Akt/bcl-2/bax/caspase-3 signaling cascades.Taken together,our research provided a promising approach for deep exploration of MFG-E8 on cytoprotection against mitochondrial injury and mitochondrial-mediated apoptosis and application of anti-apoptosis in alleviating sarcopenia.

关 键 词：MFG-E8 L6 myoblast Mitochondria CYTOPROTECTION Apoptosis 

分 类 号：TS201[轻工技术与工程—食品科学]