Non-targeted cellular metabolomics revealing the metabolomic features and anti-tumor mechanisms of cyanidin-3-O-arabinoside on Caco-2 cells  

作　　者：Ge Xu Wenjun Xue Dan Zhang Zhipeng Yu Jingbo Liu Wenzhu Zhao 

机构地区：[1]School of Food Science and Engineering,Hainan University,Haikou,570228,PR China [2]College of Food Science and Engineering,Bohai University,Jinzhou,121013,PR China [3]Lab of Nutrition and Functional Food,Jilin University,Changchun,130062,PR China

出　　处：《Food Bioscience》2023年第1期827-836,共10页食品生物科学（英文）

基　　金：supported by The China Scholarship Council(Grant No.202008210142);the Open Fund of Key Laboratory of Fruit and Vegetable Processing,Ministry of Agriculture and Rural Affairs,China(Grant No.FVKF2020002).

摘　　要：Cyanidins are considered the most widespread anthocyanin in nature,and the cyanidin-3-O-arabinoside from apple with anti-tumor activity has been screened in our previous study.However,the anti-tumor mechanism of cyanidin-3-O-arabinoside needs to be further researched.In the present study,a UPLC-Q-TOF-MS-based metabomics approach was applied to reveal the metabolite changes and metabolic pathways in Caco-2 cells after cyanidin-3-O-arabinoside treatment.The results showed 10 differential metabolites between cyanidin-3-Oarabinoside treated and untreated Caco-2 cells.Especially,sphingosine,phosphoethanolamine,and taurine were found to be significantly altered in the Caco-2 cell after cyanidin-3-O-arabinoside treatment.A comparison of the Caco-2 cell group treated with cyanidin-3-O-arabinoside versus the control group(simvastatin)revealed seven identical metabolites,including uracil,pantothenate,cytidine,phosphatidylcholine(PC),γ-linolenic acid,dodecanoic acid,and myristic acid.Cyanidin-3-O-arabinoside could exert anti-tumor effects by inducing apoptosis,reducing cell membrane synthesis and energy metabolism,and triggering DNA damage.These results provide new insights into the anti-tumor mechanism of cyanidin-3-O-arabinoside on Caco-2 cells.

关 键 词：Cyanidin-3-O-arabinoside Caco-2 cell ANTI-TUMOR Metabolomics 

分 类 号：O62[理学—有机化学]