Inhibition mechanisms of wounded okra on theα-glucosidase/α-amylase  

作　　者：Qun Yu Yu Tian Liuping Fan 

机构地区：[1]State Key Laboratory of Food Science&Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China [2]School of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China [3]Collaborat Innovat Ctr Food Safety&Qual Control,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China

出　　处：《Food Bioscience》2023年第1期1089-1099,共11页食品生物科学（英文）

基　　金：subsidized by the Jiangsu Agriculture Science and Technology Innovation Fund(CX(18)3070);the China National Post-doctoral Program for Innovative Talents(BX20220137);the Jiangsu Excellent Postdoctoral Program(2022ZB495);which has enabled us to accomplish this study.

摘　　要：Okra has attracted increasing interests to be used as functional food ingredients due to various human health benefits such as the inhibition ofα-glucosidase andα-amylase in this paper.Results showed that theα-glucosidase(andα-amylase)inhibition capacity of polyphenols in wounded okra significantly increased by 31.6%(and 28.6%)when the wounding intensity was 1.87 cm2/g.The UPLC-MS analysis indicated that quercetin and catechin derivatives were the major polyphenols in wounded okra,of which quercetin 3-O-gentiobioside and epigallocatechin dimer were the most abundant compounds.Fluorescence quenching confirmed that wound and healing process promoted the affinity betweenα-glucosidase(andα-amylase)and polyphenols,which interacted with enzymes in a mixed type manner.Molecular docking suggested that quercetin-3-O-gentiobioside,quercetin-3-O-glucosyl-xyloside,isoquercitrin,querce-tin-3-O-(malonyl)-glucoside,epigallocatechin,and epigallocatechin dimer can interact with the activity cavities of enzymes by amino acids residues(Arg195,Leu162,and His305).The results of this study support the potentials of wounded okra to inhibitα-glucosidase andα-amylase in the food industries,without associated risks to consumers.

关 键 词：Wounded okra POLYPHENOLS α-Glucosidase andα-amylase Fluorescence quenching Molecular docking 

分 类 号：TS201.4[轻工技术与工程—食品科学]