Pullulan production by Aureobasidium pullulans MTCC 1991 from apple pomace and its characterization  

作　　者：Shoib Mohmad Wani F.A.Masoodi Sajad Ahmad Mir F.A.Khanday 

机构地区：[1]Department of Biotechnology,University of Kashmir,Hazratbal Srinagar,J&K,190006,India [2]Department of Food Science&Technology,University of Kashmir,Hazratbal Srinagar,J&K,190006,India

出　　处：《Food Bioscience》2023年第1期1380-1387,共8页食品生物科学（英文）

摘　　要：Apple pomace,a by-product of the apple juice processing industry,is significant agro-based waste in the Union Territory of Jammu&Kashmir,India.A considerable amount of apple is processed for juice production,resulting in a surplus of apple pomace,which is generally underutilized.This study utilized apple pomace as a substrate matrix for producing pullulan by applying Aureobasidium pullulans MTCC 1991.Solid-state fermentation was done at a lab scale in Erlenmeyer flasks to produce this exopolysaccharide.The suitable conditions for solid-state fermentation of apple pomace were:a solid-liquid ratio of 1:4,inoculum volume of 3 mL,pH 6 and incubation time of 14 days.A yield of 42 mg/g dw was obtained in the control sample without adding any other nutrients or chemicals.The addition of yeast extract at a concentration of 1%(w/w)and sucrose(5%w/w)significantly(p<0.05)increased the yield to 62 mg/g dw and 75 mg/g dw,respectively.The purified pullulan had similar characteristics to that of standard pullulan,as confirmed by ATR-FTIR,H NMR and TLC techniques.Pullulan production from apple pomace can be a better way to utilize this by-product from the apple juice industry.It will be a cost-effective technique for pullulan production as an inexpensive substrate and an environmentally friendly approach are also used.

关 键 词：BIOPOLYMER Microbial polysaccharide Agro-based waste Solid state fermentation Waste utilization 

分 类 号：TS201[轻工技术与工程—食品科学]