熊果酸-法尼基焦磷酸合酶交联纳米粒对子宫颈癌HeLa细胞凋亡的影响及机制研究  

Effect of Ursolic Acid-farnesyl Pyrophosphate Synthase Crosslinked Nanoparticles on Apoptosis of Cervical Cancer HeLa Cells and Its Mechanism

作　　者：由艳秋 刁志宏 曹步清 谢泷宽 马贞丽 唐芳玫[1] 李福军 YOU Yanqiu;DIAO Zhihong;CAO Buqing;XIE Longkuan;MA Zhenli;TANG Fangmei;LI Fujun(Laboratory Department,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China;不详)

机构地区：[1]广西中医药大学附属瑞康医院检验科,广西南宁530011 [2]广西中医药大学附属瑞康医院手术麻醉科,广西南宁530011

出　　处：《中国医学创新》2024年第36期152-157,共6页Medical Innovation of China

基　　金：广西医疗卫生适宜技术开发与推广应用项目(S2022056)。

摘　　要：目的:探究熊果酸-法尼基焦磷酸合酶交联纳米粒(FPP@UA NPs)对HeLa细胞凋亡的影响及机制研究。方法:在2022年1月—2023年12月展开FPP@UA NPs对子宫颈癌HeLa细胞活性的探究,收集HeLa细胞株并制备纳米粒子UA NPs及FPP@UA NPs,将其分为对照组及FPP@UA NPs纳米粒载体4 μmol/L、8 μmol/L、16 μmol/L组。采用电感耦合等离子体发射光谱仪检测HeLa细胞对FPP@UA NPs的吸收量,采用流式细胞仪检测HeLa细胞凋亡情况,采用细胞计数试剂盒-8(CCK-8)检测HeLa细胞增殖情况,采用克隆形成法测定FPP@UA NPs对HeLa细胞存活的影响。结果:8 μmol/L组HeLa细胞中纳米粒子数量高于4 μmol/L组,差异有统计学意义(P<0.05);16 μmol/L组HeLa细胞中纳米粒子数量高于8 μmol/L组,差异有统计学意义(P<0.05)。与对照组比较,4 μmol/L组的HeLa细胞凋亡率升高、增殖率降低、克隆数量减少,差异均有统计学意义(P<0.05);与4 μmol/L组比较,8 μmol/L组HeLa细胞凋亡率升高、增殖率降低、克隆数量减少,差异均有统计学意义(P<0.05);与8 μmol /L组比较,16 μmol/L组HeLa细胞凋亡率升高、增殖率降低、克隆数量减少,差异均有统计学意义(P<0.05)。结论:FPP@UA NPs可能对促进子宫颈癌细胞的凋亡,抑制其克隆和增殖具有一定意义。Objective:Effect of ursolic acid-farnesyl pyrophosphate synthase crosslinked nanoparticles(FPP@UA NPs)on apoptosis of HeLa cells and its mechanism.Method:From January 2022 to December 2023,the study on FPP@UA NPs activity of HeLa cells in cervical cancer were carried out.HeLa cell lines were collected and nanoparticles UA NPs and FPP@UA NPs were prepared,and divided into control group and FPP@UA NPs nanoparticle carrier groups of 4μmol/L,8μmol/L and 16μmol/L.Inductively coupled plasma emission spectrometer was used to detect the uptake of FPP@UA NPs by HeLa cells,the apoptosis of HeLa cells was detected by flow cytometry,and the proliferation of HeLa cells was detected by cell counting kit 8(CCK-8).The effect of FPP@UA NPs on HeLa cell survival was determined by clonal formation method.Result:The number of nanoparticles in HeLa cells in 8μmol/L group was higher than that in 4μmol/L group,the difference was statistically significant(P<0.05).The number of nanoparticles in HeLa cells in 16μmol/L group was higher than that in 8μmol/L group,the difference was statistically significant(P<0.05).Compared with control group,HeLa cell apoptosis rate increased,proliferation rate decreased and number of clones decreased in 4μmol/L group,the differences were statistically significant(P<0.05).Compared with 4μmol/L group,the apoptosis rate increased,proliferation rate decreased and number of clones decreased in HeLa cells in 8μmol/L group,the differences were statistically significant(P<0.05).Compared with the 8μmol/L group,the apoptosis rate was increased,the proliferation rate was decreased, and the number of clones was decreased in HeLa cells in the 16 μmol/L group, the differences were statistically significant (P<0.05). Conclusion: FPP@UA NPs may have certain significance in promoting the apoptosis of cervical cancer cells and inhibiting their cloning and proliferation.

关 键 词：子宫颈癌 FPP@UA NPs 细胞凋亡 影响机制 

分 类 号：R737.33[医药卫生—肿瘤]