GRP-78、RRBP1在原发性肝癌组织中的表达及其与术后早期复发的关联  

Expression of GRP-78 and RRBP1 in primary liver cancer and their association with early postoperative recurrence

作　　者：邓宏哲 陈昆 张鹏 朱清海 DENG Hong-zhe;CHEN Kun;ZHANG Peng(Hepatobiliary Pancreatic and Splenic Surgery Department.Zhumadian Central Hospital,(Henan Zhumadian,463000),China.)

机构地区：[1]驻马店市中心医院肝胆胰脾外二科,河南驻马店463000

出　　处：《中西医结合肝病杂志》2024年第12期1127-1131,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探究葡萄糖调节蛋白-78(GRP-78)、核糖体结合蛋白1(RRBP1)在原发性肝癌(PLC)组织中的表达及其与术后早期复发的关联。方法:选取2018年8月至2021年8月于本院确诊并进行手术切除的PLC患者80例,术中获得癌旁组织和PLC组织。Western blot检测GRP-78、RRBP1蛋白的表达。患者出院后随访2年,依据患者是否复发分为复发组和未复发组。比较两组患者基线资料和GRP-78、RRBP1水平;采用Logistic回归分析PLC术后早期复发的影响因素;应用ROC曲线分析GRP-78、RRBP1表达水平对PLC术后早期复发的评估价值。结果:与癌旁组织相比,PLC组织中GRP-78、RRBP1的表达明显升高(P<0.05)。与未复发组相比,复发组中酗酒史、肿瘤数量、组织分化程度、TNM分期、PTL、AFP存在显著差异(P<0.05)。与未复发组相比,复发组患者PLC组织中GRP-78、RRBP1水平显著升高(P<0.05)。酗酒史、肿瘤数量、组织分化程度、TNM分期、PTL、AFP、GRP-78、RRBP1水平异常是影响AHF预后的危险因素(P<0.05)。GRP-78、RRBP1联合预测PLC患者术后早期复发的AUC、敏感度、特异度分别为0.913、91.18%、89.36%,均高于上述指标的单独预测效能(P<0.05)。结论:GRP-78、RRBP1在PLC组织中高表达,与该病患者术后早期复发关系密切,且GRP-78、RRBP1联合检测对PLC患者术后早期复发的预测价值更高。Objective:To explore the expression of GRP-78,RRBP1 in primary liver cancer(PLC)tissue and its association with early postoperative recurrence.Methods:A total of 80 PLC patients diagnosed and surgically resected in our hospital from August 2018 to August 2021 were enrolled.Intraoperative tissues and PLC tissues were obtained.The expression of GRP-78,RRBP1 protein was detected by western blot.Patients were followed up for 2 years after discharge,and were divided into relapse group and non-recurrence group.Baseline data and GRP-78,RRBP1 levels were compared between the two groups.logistic regression was used to analyze the factors affecting the early recurrence of PLC after operation.ROC curve was used to analyze the value of GRP-78 and RRBP1 expression levels in the assessment of early postoperative recurrence of PLC.Results:The expressions of GRP-78 and RRBP1 in PLC tissues were significantly increased compared with those in adjacent tissues(P<0.05).There were significant differences in alcohol abuse history,number of tumors,degree of tissue differentiation,TNM stage,PTL and AFP in relapse group compared with non-relapse group(P<0.05).The levels of GRP-78 and RRBP1 in PLC tissues of patients with recurrence were significantly increased compared with those of non-recurrence group(P<0.05).History of alcoholism,number of tumors,degree of tissue differentiation,TNM stage,abnormal level of PTL,AFP,GRP-78 and RRBP1 were the risk factors affecting the prognosis of AHF(P<0.05).The AUC,sensitivity and specificity of GRP-78 and RRBP1 combined to predict early postoperative recurrence in PLC patients were 0.913,91.18%and 89.36%,respectively,which were higher than the single prediction efficacy of the above indexes(P<0.05).Conclusion:The high expression of GRP-78 and RRBP1 in PLC tissues is closely related to the early postoperative recurrence of PLC patients,and the combined detection of GRP-78 and RRBP1 has higher predictive value for the early postoperative recurrence of PLC patients.

关 键 词：GRP-78 RRBP1 原发性肝癌 术后早期复发 LOGISTIC回归分析 

分 类 号：R735.7[医药卫生—肿瘤]