Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3,herein Sysuimicrobiota  

作　　者：Lan Liu Zheng-Han Lian Ai-Ping Lv Nimaichand Salam Jian-Chao Zhang Meng-Meng Li Wei-Min Sun Sha Tan Zhen-Hao Luo Lei Gao Yang Yuan Yu-Zhen Ming Yu-Ting OuYang Yu-Xian Li Ze-Tao Liu Chao-Jian Hu Ying Chen Zheng-Shuang Hua Wen-Sheng Shu Brian P.Hedlund Wen-Jun Li Jian-Yu Jiao 

机构地区：[1]State Key Laboratory of Biocontrol,Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China [2]National Agri-Food Biotechnology Institute,Mohali 140306,India [3]School of Earth System Science,Institute of Surface-Earth System Science,Tianjin University,Tianjin 300072,China [4]National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China,Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management,Institute of Eco-Environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China [5]State Key Laboratory of Desert and Oasis Ecology,Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China [6]Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion,Department of Environmental Science and Engineering,University of Science and Technology of China,Hefei 230026,China [7]Institute of Ecological Science,Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring,Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou 510631,China [8]Guangdong Provincial Key Laboratory of Chemical Pollution,South China Normal University,Guangzhou 510006,China [9]School of Life Sciences,University of Nevada Las Vegas,Las Vegas,NV 89154,USA [10]Nevada Institute of Personalized Medicine,University of Nevada Las Vegas,Las Vegas,NV 89154,USA

出　　处：《National Science Review》2024年第11期55-69,共15页国家科学评论（英文版）

基　　金：supported by the Key-Area Research and Development Program of Guangdong Province(2022B0202110001);the National Natural Science Foundation of China(32200010,92251302,32200007 and 32370011);the Fundamental Research Funds for the Central Universities of Sun Yat-sen University(23ptpy11,23ptpy136 and 23ptpy137);the National Science and Technology Fundamental Resources Investigation Program of China(2021FY100900);the US National Science Foundation(DEB 1557042).

摘　　要：Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood.Here,we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes,with 3 of 5 families encoding autotrophy through the reductive glycine pathway(RGP),Wood–Ljungdahl pathway(WLP)or Calvin-Benson-Bassham(CBB),with hydrogen or sulfide as electron donors.Chemoautotrophic enrichments from hot spring sediments and fluorescence in situ hybridization revealed enrichment of six CSP1-3 genera,and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms.Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP,whereas the WLP and CBB were acquired by horizontal gene transfer.Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon,sulfur and nitrogen.The name Sysuimicrobiota phy.nov.is proposed.

关 键 词：Sysuimicrobiota phy.nov. facultative anaerobes chemoautotrophic metabolisms 

分 类 号：R73-3[医药卫生—肿瘤]