lncRNA-BBOX1-2通过调控成纤维细胞生长因子受体1促进胃癌的发生和发展  

LncRNA-BBOX1-2 promotes the occurrence and progression of gastric cancer by regulating FGFR1

作　　者：孙颖[1] 顾玮[1] 王吉[1] 郑雄[1] SUN Ying;GU Wei;WANG Ji;ZHENG Xiong(Department of Gastroenterology,Luwan Branch,Ruijin Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200020 China)

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院消化内科,上海200020

出　　处：《安徽医药》2025年第1期57-62,I0002,共7页Anhui Medical and Pharmaceutical Journal

基　　金：上海市卫计委科研项目(201540572)。

摘　　要：目的探讨长链非编码RNA(long non-coding RNA,lncRNA)BBOX1-2通过调控成纤维细胞生长因子受体1(fibroblast growth factor receptor 1,FGFR1)对胃癌的发生发展机制的影响。方法回顾性选取2017年4月至2019年1月于上海交通大学医学院附属瑞金医院卢湾分院接受胃癌根治术30例病人肿瘤组织及癌旁相应正常组织作为研究对象,采用实时定量PCT(real-time PCR,RT-PCR)检测lncRNA-BBOX1-2和FGFR1表达;si-linc-BBOX1-2转染SGC-7901细胞后,通过蛋白质印迹法/细胞存活率分析(MTT)、细胞迁移和侵袭(Transwell)实验、细胞划痕、平板克隆一系列生物学功能实验,检测肿瘤细胞生物学功能及FGFR1表达的变化。结果胃癌组织中的lncRNA-BBOX1-2(3.68±0.58比1.15±0.11)和FGFR1(4.26±0.71比1.19±0.18)表达显著高于癌旁正常组织(P<0.05);si-linc-BBOX1-2转染SGC-7901细胞后,FGFR1表达下调,细胞活力、迁移、侵袭和生存能力明显下降。结论LincRNA-BBOX1-2可通过调控FGFR1的表达介导胃癌细胞的增殖、凋亡、迁移和侵袭,可能为胃癌的治疗提供了新的靶点和潜在的生物学标志物。Objective To investigate the effect of long non-coding RNA(lncRNA)BBOX 1-2 on the development mechanism of gastric cancer(GC)by regulating fibroblast growth factor receptor 1(FGFR1).Methods Tumor tissues and corresponding normal tissues adjacent GC of 30 patients who underwent radical gastrectomy in Luwan Branch of Ruijin Hospital of Shanghai Jiao Tong University School of Medicine from 4,2017 to 1,2019 were retrospectively selected as the study objects,and real-time PCR was used to detect the expression of lncRNA-BBOX1-2 and FGFR1.After SGC-7901 cells transfected with si-linc-BBOX1-2,the changes in tumor cell biological function and FGFR1 expression by a series of biological function experiments of western blotting,MTT,Transwell,cell scratches,and plate cloning were detected.Results Both lncRNA-BBOX1-2(3.68±0.58 vs.1.15±0.11)and FGFR1(4.26±0.71 vs.1.19±0.18)were overexpressed in GC tissues than those in adjacent normal tissues(P<0.05).si-linc-BBOX 1-2 transfection of SGC-7901 cells showed downregulation of FGFR1 expression and significantly decreased cell viability,migration,invasion and viability.Conclusion LincRNA-BBOX 1-2 can mediate the proliferation,apoptosis,migration and invasion of GC cells by regulating FGFR1 expression,which may provide new targets and potential biological markers for the treatment of GC.

关 键 词：胃肿瘤 长链非编码RNA BBOX1-2 成纤维细胞生长因子受体1 调控 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]