酰基辅酶A硫酯酶11在肺鳞癌中的表达及其对细胞增殖、迁移和侵袭的影响  

Expression of Acyl-CoA Thioesterases 11 in Lung Squamous Cell Carcinoma andIts Influence on Proliferation, Migration and Invasion of Cancer Cells

作　　者：刘颖[1] 赵文超 LIU Ying;ZHAO Wenchao(Department of Respiratory and Critical Care Medicine Ward,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Department of Physiology and Neurobiology,School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]郑州大学第二附属医院呼吸与危重症医学科,河南郑州450014 [2]郑州大学基础医学院生理学与神经生物学系,河南郑州450001

出　　处：《河南医学研究》2024年第24期4421-4425,共5页Henan Medical Research

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20210393)。

摘　　要：目的 明确酰基辅酶A硫酯酶11(ACOT11)在肺鳞癌(LUSC)中的表达并探讨其对LUSC细胞生物学行为的影响。方法 采用实时荧光定量PCR和生物信息学分析ACOT11在LUSC癌组织和癌旁组织中的表达。采用Kaplan-Meier法观察ACOT11的表达对患者预后的影响。将人LUSC细胞NCI-H226分为OV-ACOT11组和OV-阴性对照组,采用CCK8和Transwell法检测各组细胞的增殖、迁移和侵袭。结果 ACOT11在癌组织中的表达高于癌旁组织(P<0.05);ACOT11的高表达与患者的不良预后相关(P<0.05);过表达ACOT11促进LUSC细胞的增殖、迁移和侵袭(P<0.05)。结论 ACOT11在LUSC癌组织中表达升高,促进了LUSC细胞的增殖、迁移和侵袭,加快了LUSC的发生发展。Objective To explore the level of acyl-CoA thioesterases 11(ACOT11)in lung squamous cell carcinoma(LUSC)and observe its effect on biological behaviors of LUSC cells.Methods Quantitative real-time PCR and bioinformatical datasets were employed to examine the expression of ACOT11 in LUSC and paracancerous tissues.Kaplan-Meier was conducted to identify the effect of ACOT11 on prognosis of LUSC patients.NCI-H226 cells,a kind of human LUSC cell line,were divided into OV-ACOT11 and OV-negative control groups.CCK8 and Transwell methods were employed to observe the proliferation,migration and invasion of the cells of two groups.Results The expression of ACOT11 in cancer tissues was higher than that in adjacent tissues(P<0.05).The high expression of ACOT11 was associated with poor prognosis in patients(P<0.05).Overexpression of ACOT11 promotes proliferation,migration,and invasion of LUSC cells(P<0.05).Conclusion ACOT11 is upregulated in LUSC cancer tissue,promoting the proliferation,migration,and invasion of LUSC cells,and facilitating the occurrence and development of LUSC.

关 键 词：肺鳞癌 酰基辅酶A硫酯酶11 增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]