海参肽与高原药食两用原料复配对雄鼠运动疲劳损伤的影响  

Effect of sea cucumber peptide combined with plateau medicinal and edible raw materials on exercise-induced fatigue injury in male mice

作　　者：徐莉苹 孙绪丁 冯凤琴[3] 郑亚凤[1] 郑宝东[1] 骆贤亮 XU Li-ping;SUN Xu-ding;FENG Feng-qin;ZHENG Ya-feng;ZHENG Bao-dong;LUO Xian-liang(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Shandong Yiheng Yuan Health Technology Co.,Ltd.,Jinan 250000,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310000,China)

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]山东益衡元健康科技有限公司,济南250000 [3]浙江大学生物系统工程与食品科学学院,杭州310000

出　　处：《天然产物研究与开发》2024年第12期2122-2132,共11页Natural Product Research and Development

基　　金：福建省教育厅中青年教师教育科研(科技类)项目(JAT231021);福州市科技重大项目(2021-ZD-287);福建农林大学校企合作科研项目(KH230219A)。

摘　　要：本研究旨在探究海参肽和高原药食两用原料(牦牛鞭、鹿血、玛咖)复配对运动疲劳雄鼠的影响。采用力竭游泳构建运动疲劳雄鼠模型,将疲劳小鼠随机分为模型组、对照组(0.5 mg/g BW市售人参杜仲片)和两种剂量组合物组(0.25和0.5 mg/g BW组合物),连续干预六周,测定小鼠的脏器系数、交配能力、抗疲劳指标、组织切片、血清激素水平、精子形态及畸形率。两种剂量组合物均可改善力竭运动引起的交配能力下降,且低剂量组效果更佳;组合物可延长小鼠游泳时间,降低血清乳酸含量和肝脏丙二醛水平;低、高剂量的组合物能够有效改善力竭运动导致的血清睾酮和促黄体激素水平不足,并降低精子畸形率;睾酮、乳酸和丙二醛水平均与小鼠交配行为有显著相关性。总之,在力竭游泳训练诱导的疲劳小鼠模型中,海参肽和高原药食两用原料组合物能够改善小鼠疲劳状态和激素分泌失衡,降低生殖系统的氧化损伤。The aim of this study is to investigate the effects of the combination of sea cucumber peptide and plateau medicinal and edible raw materials(yak whip,deer blood,maca)on the reproductive health of male mice with exercise fatigue.The male model of exercise fatigue was established by acute exhaustion swimming,and the mice were randomly divided into blank group,model group,control group(0.5 mg/g BW commercially available Renshen Duzhong tablets)and two-dose composition group(0.25 and 0.5 mg/g BW formula),which were administered continuously for six weeks.The organ coefficient,mating ability,fatigue resistance index,tissue section,serum hormone level,sperm morphology and malformation rate of mice were measured.Both dosage formula improved the decreased mating ability caused by exhaustive exercise,and the low-dose group has better effects.Also,the swimming time of mice was prolonged by formula treatment,while the level of lactic acid in serum and malondialdehyde in liver were reduced.Low and high-dose formula could effectively improve insufficient serum testosterone and luteinizing hormone levels caused by exhaustive exercise,and reduce sperm deformity rates(P<0.05).In addition,testosterone,lactic acid and malondialdehyde levels were significantly correlated with the mating behavior of mice.In the exercise-induced fatigue mouse model,the combination of sea cucumber peptide and plateau medicinal and edible raw materials can improve the fatigue symptom and hormone secretion imbalance of mice,as well as reduce oxidative damage to the reproductive system.

关 键 词：海参肽 高原药食两用原料 运动疲劳 激素 疲劳损伤 

分 类 号：R283.1[医药卫生—中药学]