不同饲用植物提取物的抗氧化能力及其复合配比研究  

Study on antioxidant capacity of different forage plant extracts and their complex ratios

作　　者：庞小童 魏欣 吴妍妍[1] 聂存喜[1] PANG Xiao-tong;WEI Xin;WU Yan-yan;NIE Cun-xi

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《饲料研究》2024年第21期93-97,共5页Feed Research

基　　金：兵团重大科技项目(项目编号:2021AA004);新疆维吾尔自治区重点研发专项(项目编号:2022B02029-2)。

摘　　要：试验旨在分析不同饲用植物提取物的抗氧化能力及其复合配比。试验选取6种饲用植物提取物,以体外自由基清除率为评价指标,筛选得到抗氧化能力较强的3种植物提取物,进一步采用正交试验确定抗氧化效果最佳的复合植物提取物,并对其主要活性成分进行分析。结果显示,甜叶菊提取物、金银花提取物、黄芪提取物、甘草提取物、葡萄籽原花青素和辣椒碱均具有清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟基自由基的能力。与其他提取物相比,葡萄籽原花青素、甘草提取物和辣椒碱在较低浓度下(0.4 g/L)表现出了较强的自由基清除能力,对DPPH自由基清除能力分别达到了77.40%、92.70%、39.80%,羟基自由基清除能力分别达到了78.17%、50.21%、62.63%。葡萄籽原花青素、甘草提取物和辣椒碱以1∶1∶1(0.4∶0.4∶0.4 g/L)复配时自由基清除能力最佳。复合植物提取物中总黄酮含量为217.72 mg/g、总酚含量为111.18 mg/g、总多糖含量为10.84 mg/g、总生物碱含量为0.72 mg/g。其中,黄酮中儿茶素含量最高,酚酸中原儿茶酸含量最高。研究表明,6种植物提取物和复合植物提取物均表现出一定的抗氧化能力,葡萄籽原花青素、甘草提取物和辣椒碱配比为1∶1∶1(0.4∶0.4∶0.4 g/L)时,其抗氧化能力最强,总黄酮含量最高、其次为酚酸。The aim of this study was to analyse the antioxidant capacity of different forage plant extracts and their complex ratios.Six forage plant extracts were selected,and their antioxidant capacities were evaluated based on in vitro free radical scavenging activity.Three plant extracts with strong antioxidant capacity were identified,and orthogonal experiments were conducted to determine the best compound plant extract for antioxidant effects.Additionally,the main active components of the selected extracts were analyzed.The results showed that Stevia extract,Honeysuckle extract,Astragalus extract,Liquorice extract,grapeseed proanthocyanidins,and capsaicin possessed 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical and hydroxyl radical scavenging abilities,and compared with the other extracts,grapeseed proanthocyanidins,Liquorice extract,and capsaicin exhibited strong free radical scavenging abilities at lower concentrations(0.4 g/L),with DPPH free radical scavenging abilities of 77.40%,92.70%and 39.80%,and hydroxyl radical scavenging capacity reached 78.17%,50.21%,and 62.63%,respectively.Grapeseed proanthocyanidins,Licorice extract and capsaicin in a 1∶1∶1(0.4∶0.4∶0.4 g/L)compounded with the best free radical scavenging ability.Total flavonoids 217.72 mg/g,total phenols 111.18 mg/g,total polysaccharides 10.84 mg/g,total alkaloids 0.72 mg/g in the complex plant extract.Among the flavonoids,the highest content of catechins and the highest content of protocatechuic acid among phenolic acids.The study indicates that the six plant extracts and the composite plant extract showed some antioxidant capacity.When the ratio of grapeseed proanthocyanidins,licorice extract and capsaicin was 1∶1∶1(0.4∶0.4∶0.4 g/L),the antioxidant capacity and total flavonoid content were the highest,followed by phenolic acid.

关 键 词：植物提取物 复合配比 自由基清除 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]