Dynamic correlation between tetramethylpyrazine and influencing factors in Bacillus subtilis-fermented dehulled adlay  

作　　者：Anyan Wen Zhoujie Yang Na Liu Haiying Zeng Likang Qin 

机构地区：[1]School of Liquor and Food Engineering,Guizhou University,Guiyang,550025,China [2]College of Life Science,Guizhou University,Guiyang,China [3]Key Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province,Guiyang,550025,China [4]National&Local Joint Engineering Research Center for the Exploition of Homology Resources of Medicine and Food,Guiyang,550025,China

出　　处：《Food Bioscience》2023年第2期630-638,共9页食品生物科学（英文）

基　　金：The research was supported by the Natural Science Foundation of Guizhou Province,China(grant No.(2019)1111);Agriculture Committee of Guizhou Province,China(grant No.(2018)81);Young talent introduction program of GZU,China(grant No.(2020)60 and(2020)31).

摘　　要：Tetramethylpyrazine(TMP),a flavor compound with biological activity,naturally exists in a variety of roasted,fried or fermented foods.Biosynthesis has been green and effective practice to produce TMP,and high TMP level was obtained in Bacillus subtilis BJ3-2 fermented dehulled adlay(BDA)in previous study.To elucidate the correlation between TMP and influencing factors in this study,the dynamics of TMP and influencing factors in BDA were investigated,and the correlation between TMP and influencing factors was analyzed by Pearson’s correlation coefficients.Results showed that a weakly acidic environment was found in BDA and B.subtilis CICC 20637 fermented dehulled adlay(CDA),but a weakly alkaline environment was observed in B.subtilis BJ3-2 fermented soybean(BSB).The higher levels ofα-acetolactate decarboxylase,reducing sugar,pyruvate,diacetyl,leucine,acetoin and TMP were detected in BDA,while higher levels ofα-acetolactate synthase,free amino acids and ammonium were obtained in BSB.At the end of fermentation,the TMP content in BDA was 6.13 mg/g dry weight,which was 204-and 12-fold higher than that in BSB and CDA,respectively.TMP synthesis in BDA and CDA was remarkable correlation with pyruvate,diacetyl,leucine,ammonium and acetoin(p<0.05).In summary,a weakly acidic environment,moderate ammonium content,high leucine and acetoin levels synergistically promoted TMP efficient synthesis.

关 键 词：TETRAMETHYLPYRAZINE Dehulled adlay Bacillus subtilis Influencing factors Correlation analysis 

分 类 号：TS201.3[轻工技术与工程—食品科学]