Antifungal activity of selected lactic acid bacteria from olive drupes  

作　　者：Mario Riolo Carlos Luz Elena Santilli Giuseppe Meca Santa Olga Cacciola 

机构地区：[1]Department of Agriculture,Food and Environment,University of Catania,95123,Catania,Italy [2]Department of Agricultural Science,Mediterranean University of Reggio Calabria,89122,Reggio Calabria,Italy [3]Council for Agricultural Research and Economics,Research Centre for Olive,Fruit and Citrus Crops(CREA-OFA),87036,Rende,Cosenza,Italy [4]Laboratory of Food Chemistry and Toxicology,Faculty of Pharmacy,University of Valencia,Burjassot,Spain

出　　处：《Food Bioscience》2023年第2期959-969,共11页食品生物科学（英文）

基　　金：This study was supported by the project“Smart and innovative packaging,postharvest rot management,and shipping of organic citrus fruit(BiOrangePack)”under Partnership for Research and Innovation in the Mediterranean Area(PRIMA)-H2020(E69C20000130001);the“Italie-Tunisie Cooperation Program 2014-2020”project“PROMETEO«Un village transfrontalier pour proteger les cultures arboricoles mediterraneennes en partageant les connaissances»cod.C-5-2.1-36,CUP 453E25F2100118000;M.R.has been granted a fellowship by CREA“OFA”(Rende)MIPAAF-D.M.n.0033437 del 21/12/2017″Project 2018-2022“Salvaguardia e valorizzazione del patrimonio olivicolo italiano con azioni di ricerca nel settore della difesa fitosanitaria”-SALVAOLIVI”;This study is part of his activity as PhD,Doctorate“Agricultural,Food,and Forestry Science”,University Mediterranea of Reggio Calabria,XXXV cycle.

摘　　要：In this study,16 Lactobacilli(LABs)isolated from the drupes of olive(Olea europaea)oil varieties were identified as Lactiplantibacillus plantarum(seven isolates),Pediococcus pentosaceus(six isolates),Enterococcus faecium(two isolates)and Streptococcus salivarius(a single isolate)by peptide mass fingerprinting and sequencing of the 16S rRNA.Antifungal activity of LABs and their cell-free fermentates(CFSs)against several plant pathogenic fungi and oomycetes(fungi sensu lato),including Alternaria,Aspergillus Colletotrichum,Penicillium,Plenodomus and Phytophthora,was evaluated in vitro using the culture overlaying and the agar diffusion tests.Minimal inhibitory concentration(MIC)and minimal fungicidal concentration(MFC)were determined.LABs showed antifungal activity against the fungi sensu lato tested.The most noticeable inhibitory activity was shown by isolates of L.plantarum and P.pentosaceus against Fusarium oxysporum,Colletotrichum species and Penicillium nordicum.Chemical analysis revealed CFSs contained acid lactic and variable quantities of 14 diverse phenolic acids and 26 volatile organic compounds(VOCs).No obvious correlation was found between the metabolic profile of LABs and their antifungal efficacy.However,it is the first time that the potential of fermentates of LABs,recovered from drupes of olive oil varieties,as natural fungicides,was demonstrated.

关 键 词：LABS VOCS Organic compounds Phenolic acids Olive diseases 

分 类 号：TS201.2[轻工技术与工程—食品科学]