Insight into the phytochemical, biological, and in silico studies of Erythrina suberosa roxb.: A source of novel therapeutic bioactive products from a medicinal plant  

作　　者：Abdulhakeem S.Alamri Hammad Saleem Irfan Pervaiz Umair Khurshid Juwairiya Butt Tahir Ali Chohan Majid Alhomrani Marcello Locatelli Syafiq Asnawi Zainal Abidin Yasir Nasser H Alqahtani Kannan RRRengasamy Mohamad Fawzi Mahomoodally 

机构地区：[1]Department of Clinical Laboratory Sciences,The Faculty of Applied Medical Sciences,Taif University,Taif,Saudi Arabia [2]Centre of Biomedical Sciences Research(CBSR),Deanship of Scientific Research,Taif University,Saudi Arabia [3]Institute of Pharmaceutical Sciences(IPS),University of Veterinary&Animal Sciences(UVAS),Lahore,Pakistan [4]KITAAS,Khaldunia College of Pharmacy,Lahore,Pakistan [5]Department of Pharmaceutical Chemistry,Faculty of Pharmacy,The Islamia University of Bahawalpur,63100,Pakistan [6]School of Life Sciences,University of Westminster,115 New Cavendish Street,London,W1W 6UW,UK [7]Department of Pharmacy,University‘G.d’Annunzio of Chieti-Pescara,66100,Chieti,Italy [8]Jeffrey Cheah School of Medicine and Health Sciences,Monash University Malaysia,Jalan Lagoon Selatan,47500,Bandar Sunway,Selangor Darul Ehsan,Malaysia [9]MOI Clinics,Security Forces Hospital,Riyadh,Saudi Arabia [10]Laboratory of Natural Products and Medicinal Chemistry(LNPMC),Department of Pharmacology,Saveetha Dental College,Saveetha Institute of Medical and Technical Sciences(SIMATS),Chennai,600077,India [11]Department of Health Sciences,Faculty of Medicine and Health Sciences,University of Mauritius,R´eduit,80837,Mauritius [12]Centre of Excellence for Pharmaceutical Sciences,North-West University,Private Bag X6001,Potchefstroom,2520,South Africa

出　　处：《Food Bioscience》2023年第2期1421-1432,共12页食品生物科学（英文）

基　　金：The authors extend their appreciation to the Deputyship of Research and Innovation,Ministry of Education in Saudi Arabia for funding this research work through the project number TUIF-23A-9-5.

摘　　要：Background:Erythrina suberosa(E.suberosa)Roxb.also known as corky coral tree is used as a folklore medicine to treat ulcers and piles.This study explored the phytochemical profiles of methanol and dichloromethane(DCM)extracts from the aerial,root,and flower parts of E.suberosa.Method:Total bioactive contents,high-performance liquid chromatography array(HPLC-PDA)polyphenolic quantification,and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)analysis were used to evaluate the phytochemical profile.Biological potential was evaluated by determining their antioxidant(DPPH,ABTS,FRAP,CUPRAC,Phosphomolybdenum,and metal chelating)and enzyme inhibitory(cholinesterase,amylase,glucosidase,and tyrosinase)effects.Results:The phenolic(46.26 mg GAE/g extract)and flavonoid(32.46 mg QE/g extract)contents were found to be highest in the methanolic extract of roots(EsR-M).HPLC-PDA analysis determined the presence of numerous bioactive compounds,including catechin,syringic acid,p-hydroxy benzoic acid,gallic acid,vanillic acid,epicatechin,rutin,chlorogenic acid,carvacrol,sinapinic acid,and naringenin in considerable amounts.A total of 143 phytochemicals,belonging to phenolic,flavonoid,coumarin,terpenoid,chalcone,saponin,and glycoside classes,were tentatively identified by UHPLC-MS analysis.Aerial methanolic extract(EsA-M)exhibited highest radical scavenging(DPPH 86.81 mgTE/g extract,ABTS 245.17 mgTE/g extract)as well as reducing potential(FRAP 177.12 mgTE/g extract,CUPRAC 177.12 mgTE/g extract).Likewise,it was also noted to be most active for BChE(5.70 mg GALAE/g extract)and tyrosinase inhibition(148.35 mg KAE/g extract).While,roots-DCM extract(EsR-D)presented considerable inhibition for AChE(4.69 mg GALAE/g extract)and amylase(0.81 mmol ACAE/g extract)enzymes.Coreopsin,davallioside A,and isovitexin were docked to tyrosinase to depict possible interaction.Conclusion:This study has established baseline data on the biological and phytochemical profile of E.suberosa which was found to possess antioxidant and enzym

关 键 词：Erythrina suberosa HPLC-PDA UHPLC-MS Antioxidant Enzyme inhibition Natural products 

分 类 号：TS201.2[轻工技术与工程—食品科学]