Vitamin and zeatin treatments promote colchicine-induced haploid chromosome doubling in maize  

作　　者：Chen Chen Yuling Zhang Chenxu Liu Jinlong Li Jiuran Zhao Yuandong Wang Shaojiang Chen 

机构地区：[1]National Maize Improvement Center of China,College of Agronomy and Biotechnology,Sanya Research Institute,China Agricultural University,Beijing 100193,China [2]Maize Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China

出　　处：《The Crop Journal》2024年第6期1677-1685,共9页作物学报（英文版）

基　　金：supported by the Science and Technology Innovation 2030-Major Project (2023ZD0403001);China Agriculture Research System (CARS-02);Beijing Academy of Agriculture and Forestry Sciences Excellent Scientist Training Program (JKZX202202);National Natural Science Foundation of China (32001554);Beijing Academy of Agriculture and Forestry Sciences Science and Technology Innovation Capability Improvement Project (KJCX20230103);Chinese Universities Scientific Fund (2022TC141).

摘　　要：Doubled haploid(DH)technology is an efficient method used in commercial maize breeding.Chromosome doubling is a vital step of DH technology;however,the underlying processes regulating chromosome doubling of haploid is still not well understood,which is key to optimize the technology.In this study,the immature haploid embryos of the maize inbred line Zheng58 treated with amiprophos-methyl(APM)or colchicine were used to analyze transcriptomic and metabolomic changes,75 and 60 differential expressed metabolites(DEMs)were identified between control treatment,respectively.Most differentially expressed genes(DEGs)related to artificial chromosome doubling were down regulated;these were mainly involved in mitosis process.Both DEMs and DEGs co-expression analyses showed that,compared to controls,zeatin biosynthesis and cofactor and vitamin metabolism were significantly enriched in both APM and colchicine treatments.In a parallel experiment,exogenous vitamins including thiamine,nicotinic acid,vitamin B6,or trans-zeatin were added to colchicine treatment;there were synergistic effects between vitamins or zeatin and colchicine in haploid artificial chromosome doubling.These results provide novel insights in exploring the molecular responses to antimitotic reagents at both the transcriptomic and metabolomic levels.In addition,the application efficiency of haploid breeding will be greatly improved by the key factors for artificial chromosome doubling.

关 键 词：Antimitotic reagent Artificial chromosome doubling TRANSCRIPTOMIC Metabolomic MAIZE 

分 类 号：S513[农业科学—作物学]