Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut  

作　　者：Kunkun Zhao Xufa Du Jingjing Zhang Yi Fan Qian Ma Yaoyao Li Zenghui Cao Di Cao Sasa Hu Ding Qiu Kai Zhao Fangping Gong Zhongfeng Li Rui Ren Lin Zhang Xingli Ma Xingguo Zhang Dongmei Yin 

机构地区：[1]College of Agronomy&Peanut Functional Genome and Molecular Breeding Engineering,Henan Agricultural University,Zhengzhou 450000,Henan,China

出　　处：《The Crop Journal》2024年第6期1745-1753,共9页作物学报（英文版）

基　　金：supported by the Key Program of National Natural Science Foundation of China (NSFC)-Henan United Fund (U22A20475);Key Scientific and Technological Project of Henan Province (221111110500,222301420026,HARS-22-05-G1).

摘　　要：Pod size is a key agronomic trait that influences peanut yield greatly.However,our understanding of the mechanisms underlying pod size is limited.In this study,we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci(QTL)associated with pod size.Initial mapping performed using bulk segregant analysis revealed a candidate interval on chromosome A05 referred to as qPSW05.We refined this interval to a 256.9 kb genomic region using newly developed molecular markers.Through sequence and expression analyses,we identified the candidate gene AhXE45GC,which encodes an AN1 zinc finger protein.We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S.Accessions that lack this insertion,such as ND_L,had significantly larger pods than those with the insertion,including ND_S.To facilitate marker-assisted selection for peanut pod size,we developed a molecular marker associated with this polymorphism.This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.

关 键 词：PEANUT Pod size BSA-seq Zinc finger protein 

分 类 号：S565.2[农业科学—作物学]