FOXM1c调控选择性自噬接头蛋白p62启动子活性的研究  

FOXM1c regulates the promoter activity of selective autophagy adaptor protein p62

作　　者：顾超 杨家龙 舒超 徐芳[1] GU Chao;YANG Jialong;SHU Chao;XU Fang(College of Medicine,Tarim University,Alar,Xinjiang 843300;The First People’s Hospital of Korla,Korla,Xinjiang 841000)

机构地区：[1]塔里木大学医学院,新疆阿拉尔843300 [2]新疆维吾尔自治区库尔勒市第一人民医院,新疆库尔勒841000

出　　处：《塔里木大学学报》2024年第4期98-104,共7页Journal of Tarim University

基　　金：塔里木大学校长基金硕士人才项目(TDZKSS202263)。

摘　　要：为研究转录因子FOXM1c对自噬相关蛋白p62启动子活性的影响。通过生物信息学软件预测转录因子FOXM1c和p 62基因启动子的结合位点,合成p 62基因野生型的启动子片段和突变型p 62基因DNA片段,并分别构建含有p 62野生型和突变型启动子片段的荧光素酶报告基因载体;构建pcDNA3.1-FOXM1c-EGFP表达载体;用双荧光素酶报告基因检测系统分析FOXM1c对野生型和突变型p 62基因启动子转录活性的影响。结果表明,构建的野生型和突变型的p 62启动子均具有转录活性,转录因子FOXM1c与野生型的p 62基因启动子存在负调控作用,突变后这种相互作用消失。To study the regulatory effects of transcription factor FOXM1c on the promoter activity of autophagy-related protein p62.Bioinformatics software was used to predict the binding sites of transcription factors FOXM1c and the promoter region of p 62.The wild-type promoter fragment of p 62 gene and the mutant p 62 gene DNA fragment were synthesized,and the luciferase reporter gene vectors containing p 62 wild-type and mutant promoter fragments were constructed respectively.The pcDNA3.1-FOXM1c-EGFP expression vector was constructed.The effect of FOXM1c on the transcriptional activity of the wild-type and the mutant p 62 gene promoter was evaluated using the dual luciferase reporter gene detection system.The findings indicate that both the wild-type and mutant p 62 promoters demonstrate transcriptional activity.Furthermore,the transcription factor FOXM1c was observed to have a negative effect on the wild-type p 62 gene promoter,however,this negative effect became undetectable in the mutated-type p 62 gene promoter.

关 键 词：FOXM1c P62 启动子 细胞自噬 

分 类 号：R394.3[医药卫生—医学遗传学]