miR-125b-5p、miR-1207-3p、miR-802在前列腺癌组织中的表达及其与临床病理特征和预后的关系  

Expression of miR-125b-5p,miR-1207-3p,and miR-802 in prostate cancer tissues and their relationship with clinicopathologic features and prognosis

作　　者：吴远博 龙慧民[1] WU Yuanbo;LONG Huimin(Department of Urology,Ningbo Medical Center Li Huili Hospital,Ningbo 315040,China)

机构地区：[1]宁波市医疗中心李惠利医院泌尿外科,浙江宁波315040

出　　处：《空军军医大学学报》2024年第12期1446-1451,1456,共7页Journal of Air Force Medical University

基　　金：浙江省医药卫生科技计划项目(2024KY299);宁波市临床重点专科建设项目(2023-BZZ);宁波市医疗中心李惠利医院重点培育学科建设计划项目(2022-P09)。

摘　　要：目的分析前列腺癌(PCa)组织中miR-125b-5p、miR-1207-3p、miR-802表达水平,并探析指标与病理参数、预后的关系。方法选取161例PCa患者作为研究对象,病例收集时间为2019年1月至2021年7月,收集地点为宁波市医疗中心李惠利医院。患者从确诊当日起开始随访,随访时间截至2024年7月,随访期间失访3例,最终纳入158例进行研究,根据预后情况分为预后不良组(53例)和预后良好组(105例)。收集PCa患者手术切除的新鲜癌组织和癌旁组织标本,比较癌组织和癌旁组织miR-125b-5p、miR-1207-3p、miR-802表达,分析PCa患者癌组织miR-125b-5p、miR-1207-3p、miR-802表达与临床病理特征的关系,比较不同预后PCa患者癌组织miR-125b-5p、miR-1207-3p、miR-802表达。应用Cox回归分析PCa患者不良预后的危险因素,并将预后不良组纳入阳性,预后良好组纳入阴性,分析癌组织miR-125b-5p、miR-1207-3p、miR-802对PCa患者不良预后的预测价值,应用受试者工作特征曲线(ROC)获取曲线下面积(AUC)。结果PCa患者癌组织miR-125b-5p、miR-802表达低于癌旁组织(P<0.05),癌组织miR-1207-3p表达高于癌旁组织(P<0.05)。Gleason评分>7分、Ⅲ~Ⅳ期、有淋巴结转移的PCa患者癌组织miR-125b-5p、miR-802表达低于Gleason评分≤7分、Ⅰ~Ⅱ期、无淋巴结转移者(P<0.05);而癌组织miR-1207-3p表达高于Gleason评分≤7分、Ⅰ~Ⅱ期、无淋巴结转移者(P<0.05)。预后不良组中癌组织miR-125b-5p、miR-802表达低于预后良好组(P<0.05),癌组织miR-1207-3p表达高于预后良好组(P<0.05)。Cox回归分析可见,癌组织miR-125b-5p表达偏低(HR=1.091)、miR-1207-3p表达偏高(HR=1.099)、miR-802表达偏低(HR=1.135)与PCa患者不良预后有关(P<0.05)。ROC分析可见,癌组织miR-125b-5p、miR-1207-3p、miR-802联合检测预测PCa患者不良预后的AUC值为0.900,高于三者单一检测(AUC值分别为0.689、0.679、0.758,P<0.05)。结论PCa组织中miR-125b-5p、miR-802呈低表达,而miR-1207-Objective To analyze the expression levels of miR-125b-5p,miR-1207-3p,and miR-802 in prostate cancer(PCa)tissues,and to explore the relationship between the indicators and pathological parameters and prognosis.Methods A total of 161 PCa patients were selected as the study subjects,and the cases were collected from January 2019 to July 2021 at Ningbo Medical Center Li Huili Hospital.The patients were followed up from the day of diagnosis until July 2024.During the follow-up period,3 cases were lost to follow-up,and 158 cases were finally included for the study.According to the prognosis,they were divided into the poor prognosis group(53 cases)and the good prognosis group(105 cases).Fresh cancer tissues and paracancerous tissue specimens surgically removed from patients with PCa were collected,and the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues and paracancerous tissues were compared.The relationship between the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues and clinicopathological features of PCa patients was analyzed,and the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues of PCa patients with different prognosis were compared.The risk factors for poor prognosis of PCa patients were analyzed by Cox regression,and the poor prognosis group was included in the positive and the good prognosis group was included in the negative.The predictive value of miR-125b-5p,miR-1207-3p,and miR-802 of cancer tissues on the poor prognosis of PCa patients was analyzed.Area under the curve(AUC)was obtained using receiver operating characteristic curve(ROC).Results The expression of miR-125b-5p and miR-802 in cancer tissues of PCa patients was lower than that in paracancerous tissues(P<0.05),and the expression of miR-1207-3p in cancer tissues was higher than that in paracancer tissues(P<0.05).The expression of miR-125b-5p and miR-802 in cancer tissues of PCa patients with Gleason score>7,stageⅢⅣ,and lymph node metastasis was lower than that in those with Gleason scor

关 键 词：前列腺癌 miR-125b-5p miR-802 病理特征 预后 危险因素 预测价值 

分 类 号：R737.25[医药卫生—肿瘤]