川崎病Sema4D/IL-6轴介导中性粒细胞和内皮细胞相互作用的研究  

Study of Sema4D/interleukin-6 axis mediated interaction between neutrophils and endothelial cells in Kawasaki disease

作　　者：王雪蓉 张笑 唐欣妮 周新阳 张书婉 黄君华 WANG Xuerong;ZHANG Xiao;TANG Xinni;ZHOU Xinyang;ZHANG Shuwan;HUANG Junhua(School of Medical Technology,Xi'an Medical University,Xi'an 710021,Shaanxi,China;Department of Clinical Laboratory,Xi'an Children's Hospital,Xi'an 710003,Shaanxi,China)

机构地区：[1]西安医学院医学技术学院,陕西西安710021 [2]陕西省西安市儿童医院检验科,陕西西安710003

出　　处：《右江医学》2024年第11期979-982,共4页Chinese Youjiang Medical Journal

基　　金：陕西省大学生创新创业训练计划项目(S202311840077,S202311840070);西安医学院大学生创新创业训练计划项目(121523077,121523070)。

摘　　要：目的探讨信号素4D(Sema4D)/白细胞介素6(IL-6)轴在川崎病(KD)中性粒细胞与内皮细胞间的相互作用。方法选取55例急性期KD患儿作为KD组,30例健康儿童作为HC组,ELISA法检测两组外周血Sema4D和IL-6浓度,流式细胞术检测中性粒细胞表面Sema4D表达;Sema4D体外刺激内皮细胞并检测IL-6表达;用内皮细胞条件培养基处理中性粒细胞后检测Sema4D脱落情况。结果与健康儿童比较,急性期KD患儿外周血Sema4D和IL-6明显升高,而Sema4D high中性粒细胞明显降低,差异有统计学意义(P<0.05);与未刺激组比较,Sema4D刺激组的内皮细胞IL-6表达显著增加,差异有统计学意义(P<0.05);与未刺激组相比,Sema4D处理组的内皮细胞条件培养基显著增加中性粒细胞Sema4D的脱落(P<0.01)。结论川崎病冠脉病变过程中可能存在由Sema4D/IL-6轴介导的中性粒细胞和内皮细胞互作方式。Objective To explore the role of semaphorin 4D(Sema4D)/interleukin-6(IL-6)axis in interaction between neutrophils and human coronary artery endothelial cells(HCAECs)in Kawasaki disease(KD).Methods A total of 55 patients with acute KD were selected as KD group,and 30 healthy children were selected as HC group.The concentrations of Sema4D and IL-6 in peripheral blood of the two groups were detected by ELISA,and the expressions of Sema4D on the surface of neutrophils were detected by flow cytometry;Sema4D was used to stimulate endothelial cells in vitro and the expressions of IL-6 were detected;after treating neutrophils with endothelial cell-conditioned medium,the shedding of Sema4D was detected.Results Compared with healthy children,Sema4D and IL-6 in peripheral blood in the acute phase of KD significantly increased,while the Sema4D high neutrophils significantly decreased,and difference was statistically significant(P<0.05).Compared with unstimulated group,the expressions of IL-6 in endothelial cells stimulated by Sema4D significantly increased,and difference was statistically significant(P<0.05).Compared with unstimulated group,Sema4D-treated group's endothelial cell-conditioned medium significantly increased the shedding of Sema4D in neutrophils(P<0.01).Conclusion There may be a Sema4D/IL-6 axis mediated interaction between neutrophils and endothelial cells during the process of coronary artery disease in KD.

关 键 词：信号素4D 白细胞介素 川崎病 中性粒细胞 冠状动脉内皮细胞 

分 类 号：R737.11[医药卫生—肿瘤]