青海草地早熟禾全长转录组测序及功能分析  

Full-length Transcriptome Sequencing and Functional Analysis of Poa Pratensis L.cv.Qinghai

作　　者：慕星宇 刘颖 乔志梅 赵洪奎 鲍根生[2] MU Xingyu;LIU Ying;QIAO Zhimei;ZHAO Hongkui;BAO Gensheng(Qinghai Key Laboratory of Alpine Grassland Adaptive Management,Qinghai Academy of Animal Science and Veterinary Science,Qinghai University,Xining 810006,China;Qinghai Key Laboratory of Excellent Forage Germplasm Resources Utilization on Qinghai-Tibet Plateau,Academy of Animal Science and Veterinary Science,Qinghai University,Xining 810006,China;Agriculture and Animal Husbandry Comprehensive Service Center of Guoluo,Qinghai Province,Guoluo 814000,China)

机构地区：[1]青海大学青海省畜牧兽医科学院青海省高寒草地适应性管理重点实验室,青海西宁810006 [2]青海大学畜牧兽医科学院青海省青藏高原优良牧草种质资源利用重点实验室,青海西宁810006 [3]青海省果洛州农牧综合服务中心,青海果洛814000

出　　处：《高原科学研究》2024年第4期43-54,共12页Plateau Science Research

基　　金：国家自然科学基金项目(U21A20241);青海省科技特派员专项项目(2024-NK-P65)。

摘　　要：青海草地早熟禾(Poa pratensis L.cv.Qinghai)是一种优质的多年生冷季生态草,被广泛应用于生态修复、城市绿化等领域。该研究利用PacBio Iso-Seq测序技术,对青海草地早熟禾进行了全长转录组测序,并对其进行了结构分析和功能注释。研究共获得37613条全长转录本,其中95.24%的转录本在NR、GO、KEGG等数据库中得到了注释。功能注释结果显示,转录本主要与新陈代谢、信号传导等通路相关。此外,研究还预测了8750个转录因子,为进一步解析青海草地早熟禾产量形成机制提供了重要资源。Poa pratensis L.cv.Qinghai is a high-quality perennial cool-season ecological grass that has been widely used in ecological restoration,urban greening and other fields.In this study,the full-length transcriptome sequencing was performed on Poa pratensis and structural analysis and functional annotation was conducted on it by bioinformatics analysis.A total of 37613 full-length transcripts were obtained,of which 95.24%was annotat-ed in databases such as NR,GO,KEGG and so on.The results of functional annotation indicated that that the transcript is mainly related to pathways of metabolism and signal transduction.Additionally,8750 transcription factors were predicted providing important resources for further elucidating the yield formation mechanism of Poa Pratensis L.cv.Qinghai.

关 键 词：青海草地早熟禾 全长转录组 三代测序 功能注释 

分 类 号：S812.4[农业科学—草业科学]