甘松挥发油提取工艺优化及其化学成分研究  

Optimization of the Extraction Process of Volatile Oil of Nardostachys jatamansi and Comparative Analysis of Its Chemical Constituents by GC-MS and SPMEGC-MS Methods

作　　者：张郝男 贺奇峰 刘玉仙 班玛杨措 角杰本 林鹏程 Zhang Haonan;He Qifeng;Liu Yuxian;Banma Yangcuo;Jiao Jieben;Lin Pengcheng(College of Pharmacy,Qinghai Minzu University,Xining 810007,China;Qinghai Key Laboratory of Phytochemistry on Qinghai-Xizang Plateau,Xining 810007,China;Key Laboratory of Tibetan Medicine Resources Protection and Development on Qinghai-Tibet Plateau,State Ethnic Affairs Commission,Xining 810007,China)

机构地区：[1]青海民族大学药学院,西宁810007 [2]青海省青藏高原植物化学重点实验室,西宁810007 [3]国家民委青藏高原藏药资源保护与开发重点实验室,西宁810007

出　　处：《青海科技》2024年第5期35-47,共13页Qinghai Science and Technology

基　　金：青海省科技成果转化专项项目(2024-SF-131);青海省应用基础研究项目(2022-ZJ-739);青海省药材资源保护与高值化利用创新团队项目(2021XJPI02);青海省创新平台建设项目(2022-ZJ-Y14)。

摘　　要：目的:优化甘松挥发油提取工艺的同时检测其挥发性成分。方法:通过单因素试验考察加盐量、料液比、浸泡时间和提取时间对甘松挥发油含量的影响,选定工艺参数和变化水平。采用Box-Benhnken响应面法,以甘松挥发油含量为评价指标,建立数学回归模型。采用气象色谱-质谱(GC-MS)和顶空进样微萃取-气相色谱-质谱联用(SPME-GC-MS)分析甘松挥发油成分。结果:根据Box-Benhnken响应面优化设计试验结果和拟合的回归方程,采用Design Expert 11软件拟合后得到最优挥发油提取工艺参数为提取时间10.158 h、料液比1∶26.476、加盐量为3.016%、粉碎粒径为43.306目,在此条件下甘松挥发油提取率为4.278%。GC-MS分析甘松挥发油共有62种成分,顶空进样微萃取-气质联用分析出38种成分。结论:在该提取实验条件下甘松挥发油提取率稳定,GC-MS分析条件下可分析到较理想的挥发油成分。Objective:To optimize the extraction process of volatile oil from Nardostachys jatamansi and to analyze its volatile components.Methods:The effects of salt addition,material-liquid ratio,soaking time,and extraction time on the content of N.jatamansi volatile oil were investigated through single-factor experiments.The Box-Behnken response surface method was employed to establish a mathematical regression model,using the content of N.jatamansi volatile oil as the evaluation index.Gas chromatography-mass spectrometry(GC-MS)and solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)were utilized to analyze the components of the volatile oil.Results:Based on the optimization results from the Box-Behnken response surface design and the fitted regression equation,the optimal extraction parameters were determined to be an extraction time of 10.158 hours,a material-liquid ratio of 1:26.476,a salt addition of 3.016%,and a grinding granularity of 43.306 mesh.Under these conditions,the yield of N.jatamansi volatile oil was 4.278%.A total of 62 components were identified by GC-MS analysis,and 38 components were identified by SPME-GC-MS analysis.Conclusion:Under these extraction experimental conditions,the extraction rate of N.jatamansi volatile oil was stable,and the GC-MS analysis conditions allowed for the analysis of a more desirable range of volatile oil components.

关 键 词：甘松 挥发油 响应面法 GC-MS SPME-GC-MS 

分 类 号：R284.2[医药卫生—中药学] TQ654.2[医药卫生—中医学]