基于单细胞测序技术探讨动物骨骼肌卫星细胞与生态位细胞之间的“对话”  

Explore the"Cross-talk"between Skeletal Muscle Satellite Cells and the Niche Cells Based on Single-cell Sequencing Technology

作　　者：曹官从 马露 任灵芝 李杨[1] 史新娥[1] 杨公社[1] 李晓[1] CAO Guancong;MA Lu;REN Lingzhi;LI Yang;SHI Xin’e;YANG Gongshe;LI Xiao(Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;Gushi County Center for Animal Disease Control and Prevention,Gushi 465200,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西省动物遗传育种与繁殖重点实验室,杨凌712100 [2]河南省固始县动物疾病预防控制中心,固始465200

出　　处：《畜牧兽医学报》2024年第12期5340-5348,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技创新2030—重大项目(2023ZD0404702)。

摘　　要：单细胞测序技术是在单个细胞水平上对基因组、转录组、表观组进行高通量测序分析的一项前沿技术。它能够揭示细胞群体差异和细胞发育谱系关系,反映细胞群体的异质性及细胞间的相互作用,在肿瘤发生、干细胞生物学等多个领域发挥重要作用。动物骨骼肌组织是以肌纤维为主,包含肌卫星细胞、成纤维-成脂祖细胞和巨噬细胞等多种细胞在内的非均质组织。受益于单细胞测序技术的迅速发展,人们对骨骼肌中细胞群体组成、不同群体之间互作的了解逐渐加深。本文围绕骨骼肌中细胞群体组成及细胞间互作,聚焦肌卫星细胞与生态位细胞的“交叉对话”在调控骨骼肌生长、代谢及再生等过程中的作用,对已有的研究成果进行简单的综述,以期为调控产肉动物生长及改善肉质提供新思路。Single-cell sequencing is a cutting-edge technology for high-throughput sequencing of genomes,transcriptomes,and epigenomes of individual cells,offering a powerful tool to characterize individual cells and elucidate interactions between cells,and has provided many new insights in tumorigenesis and stem cell biology.Mammalian skeletal muscle is a heterogeneous tissue dominated by myofibers,including various kinds of cells such as muscle satellite cells,fibro-adipogenic progenitors and macrophages,et al.Benefiting from the rapid development of single-cell sequencing technology,the understanding of the composition of cell populations and the interactions between different kinds of cells within skeletal muscle has gradually expanded.Here,the biological meanings of the interaction of muscle satellite cells with the niche cells on the growth,metabolism and regeneration of skeletal muscle has been reviewed,with the aim to provide new ideas to improve the growth rate and meat quality of meat-producing animals.

关 键 词：骨骼肌 单细胞测序 肌卫星细胞 细胞通讯 

分 类 号：S852.2[农业科学—基础兽医学]