单细胞测序技术解析上皮性卵巢癌免疫微环境的研究进展  

Research Progress on Utilizing Single-Cell Sequencing Technology to Investigate Tumor Immune Microenvironment in Epithelial Ovarian Cancer

作　　者：李丹宁 汪希鹏(审校) Dan-ning;WANG Xi-peng(Department of Obstetrics and Gynecology,Xinhua Hospital Afiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院妇产科,200092

出　　处：《国际妇产科学杂志》2024年第6期654-658,共5页Journal of International Obstetrics and Gynecology

摘　　要：上皮性卵巢癌(epithelial ovarian cancer,EOC)是女性生殖系统恶性肿瘤中致死率最高的疾病。肿瘤免疫微环境中,免疫细胞与肿瘤细胞的相互作用可能形成恶性循环,促进肿瘤发展。因此,深入探索肿瘤免疫微环境对制定EOC的免疫治疗方案至关重要。传统高通量测序技术仅能检测细胞群体平均的基因表达水平,难以捕捉稀有异质性细胞,限制了对复杂肿瘤免疫微环境的全面理解。单细胞测序技术的出现突破了这一限制,通过高分辨率测序实现了从单细胞水平揭示肿瘤免疫微环境的异质性,这项技术能够精确识别不同免疫细胞亚群,分析其发育分化路径,并探究细胞间的相互作用。本综述还特别关注了EOC多部位的免疫微环境特征,总结了单细胞测序技术在指导个体化免疫治疗中的应用前景,并指出了未来研究方向,为促进EOC的免疫治疗提供重要参考。Epithelial ovarian cancer(EOC)has the highest mortality rate among gynecological malignancies.The interactions between immune cells and tumor cells in the tumor immune microenvironment may form a vicious cycle,promoting tumor development.Therefore,in-depth exploration of the tumor immune microenvironment is crucial for developing immunotherapy strategies for EOC.Traditional high-throughput sequencing techniques can only detect average gene expression levels of cell populations,masking rare heterogeneous cells and limiting our comprehensive understanding of the complex tumor immune microenvironment.The advent of single-cell sequencing technology has overcome this limitation,revealing the heterogeneity of the EOC immune microenvironment at the individual cell level through high-resolution sequencing.This technology enables precise identification of different immune cell subsets,analysis of their developmental and differentiation pathways,and investigation of intercellular interactions.This review particularly focuses on the immune microenvironment characteristics of multiple sites in EOC,summarizes the application prospects of single-cell sequencing in guiding personalized immunotherapy and developing novel treatment strategies,and points out future research directions,providing important references for advancing immunotherapy.

关 键 词：卵巢上皮癌 肿瘤微环境 抗肿瘤药 免疫 免疫疗法 单细胞分析 序列分析 RNA 

分 类 号：R737.31[医药卫生—肿瘤]