基于人胚胎干细胞体外模型评价化合物胚胎毒性的研究进展  

Progress in Evaluating Embryotoxicity of Compounds Based on Human Embryonic Stem Cell Model in Vitro

作　　者：孙璐 陈然然 宋殿荣(审校)[2] SUN Lu;CHEN Ran-ran;SONG Dian-rong(Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Department of Gynecology,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China)

机构地区：[1]天津中医药大学研究生院,301617 [2]天津中医药大学第二附属医院妇科

出　　处：《国际妇产科学杂志》2024年第6期707-711,共5页Journal of International Obstetrics and Gynecology

摘　　要：胚胎干细胞试验(embryonic stem cell test,EST)近年来被广泛应用于药物、化工、环境安全等领域的化合物的胚胎毒性或发育毒性评价。EST相比传统生殖毒性试验来说具有周期短、高通量、低损耗等优势,为了进一步提高EST对各种化合物的适用性和安全性评估以及避免种属差异,研究者们使用人胚胎干细胞(human embryonic stem cell,hESC)代替最初使用的小鼠胚胎干细胞(mouse embryonic stem cell,mESC);并且利用hESC进行胚胎毒性或发育毒性评估的研究越来越多样化和深入,如开发不同分化类型的评价模型(心肌、神经和成骨细胞等)或者构建更接近胚胎真实生存条件的三维模型(拟胚体和类器官),这些研究为建立更加准确和高效的胚胎毒性评价模型提供了新的方向。Embryonic stem cell test(EST)was widely used in evaluation of embryotoxicity or developmental toxicity of compounds in the fields of drugs,chemicals,and environmental safety.In recent years.Compared to the traditional reproductive toxicity tests,EST has the advantages of short cycletime,high throughput and low loss,in order to further improve the applicability and safety assessment of EST to various drugs and to avoid species differences,researchers used human embryonic stem cell(hESC)instead of the original mouse embryonic stem cell(mESC),and the researches which utilized hESC to evaluate embryotoxicity or developmental toxicity have been became more diversified and in-depth,such as the development of different evaluation models of differentiation types(myocardial cell,nerve cell and osteoblast etc.)or the construction of three-dimensional(3D)models which were closer to real living condition of embryo(embryoid bodys and organoid),these studies provided a new direction for establishing a more accurate and efficient model for the evaluation of embryotoxicity.

关 键 词：人胚胎干细胞 胚胎 哺乳动物 模型 理论 细胞 培养的 体外培养技术 胚胎毒性 

分 类 号：R-33[医药卫生]