Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging  

作　　者：Chang Liu Wilhelm Eschen Lars Loetgering Daniel S.Penagos Molina Robert Klas Alexander Iliou Michael Steinert Sebastian Herkersdorf Alexander Kirsche Thomas Pertsch Falk Hillmann Jens Limpert Jan Rothhardt 

机构地区：[1]Institute of Applied Physics,Abbe Center of Photonics,Friedrich-Schiller-University Jena,Albert-Einstein-Straße 15,07745 Jena,Germany [2]Helmholtz-Institute Jena,Fröbelstieg 3,07743 Jena,Germany [3]GSI Helmholtzzentrum für Schwerionenforschung,Planckstraße 1,64291 Darmstadt,Germany [4]Present address:Carl Zeiss AG,Carl-Zeiss-Promenade 10,07745 Jena,Germany [5]Leibniz Institute for Natural Product Research and Infection Biology,Hans Knöll Institute(Leibniz-HKI),Adolf-Reichwein-Str.23,07745 Jena,Germany [6]Department of Pharmaceutical Microbiology,Hans Knöll Institute(HKI),Friedrich-Schiller-University Jena,Winzerlaer Straße 2,07745 Jena,Germany [7]Fraunhofer Institute for Applied Optics and Precision Engineering,Albert-Einstein-Str.7,07745 Jena,Germany [8]Present address:Biochemistry/Biotechnology,Faculty of Engineering,Hochschule Wismar University of Applied Sciences Technology,Business and Design,Philipp-Müller-Str.14,23966 Wismar,Germany

出　　处：《PhotoniX》2023年第1期605-619,共15页智汇光学（英文）

基　　金：Strategy and Innovation Grant from the Free State of Thuringia(41-5507-2016);Innovation Pool of the Research Field Matter of the Helmholtz Association of German Research Centers(project FISCOV);Leibniz Research Cluster InfectoOptics(SAS-2015-HKI-LWC);Thüringer Ministerium für Bildung,Wissenschaft und Kultur(2018 FGR 0080);Helmholtz Association(incubator project Ptychography 4.0);Fraunhofer-Gesellschaft(Cluster of Excellence Advanced Photon Sources);German Research Foundation(Deutsche Forschungsgemeinschaft,DFG)under Germany’s Excellence Strategy–EXC 2051–Project-ID 390713860;S.H.is supported by the German Research Foundation(Deutsche Forschungs-gemeinschaft,DFG)–SFB 1127/2 ChemBioSys–239748522.

摘　　要：Table-top extreme ultraviolet(EUV)microscopy offers unique opportunities for label-free investigation of biological samples.Here,we demonstrate ptychographic EUV imaging of two dried,unstained model specimens:germlings of a fungus(Aspergillus nidulans),and bacteria(Escherichia coli)cells at 13.5 nm wavelength.We find that the EUV spectral region,which to date has not received much attention for biological imaging,offers sufficient penetration depths for the identification of intracellular features.By implementing a position-correlated ptychography approach,we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest.The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens.Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.

关 键 词：EUV PTYCHOGRAPHY MICROORGANISM HHG 

分 类 号：TN23[电子电信—物理电子学]