Comparison of the effects and mechanisms of Alismatis Rhizoma with and without salt processing in ameliorating edema due to kidney Yin deficiency based on UPLC-Q-TOF-MS and transcriptomics  

作　　者：Lin Yan Zemin Ou Yi Cheng Yan Tong Javed Iqbal Jinyu Wang Dewen Liu 

机构地区：[1]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China [2]Department of Biological Sciences,Govt.Graduate College Bhakkar Affiliated with University of Sargodha,Punjab,Pakistan

出　　处：《Science of Traditional Chinese Medicine》2024年第2期169-179,共11页中医药科学（英文）

基　　金：funded by the National Natural Science Foundation of China(Grant No.82003951).

摘　　要：Salt-processed Alismatis Rhizoma(SAR)is extensively used in clinical practice and exhibits a more robust urination-promoting effect than Alismatis Rhizoma(AR).This study employed ultra-performance liquid chromatography–quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)and pattern analysis to compare the terpenoids between AR and SAR.Additionally,this study compared the effects of AR and SAR on the gene expression in the kidneys of the rat model of edema(syndrome of kidney Yin deficiency)by transcriptomics to decipher the mechanism of salt processing.Materials and methods:AR and SAR were extracted by ultrasonication,and data were collected by UPLC-Q-TOF-MS in the positive ionmode.Transcriptome sequencing was employed to determine the gene expression levels of the ratmodel treated with AR and SAR,and the differentially expressed genes(DEGs)were obtained.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment was performed for the DEGs.The protein-protein interaction(PPI)network was built,on the basis of which the core targets were screened out.Finally,real-time quantitative polymerase chain reaction(RT-PCR)was conducted to verify the core targets.Results:A total of 63 terpenoids were detected in AR and SAR,and salt processing had a significant effect on the content of terpenoids.AR and SAR mainly participated in the regulation of inflammation and immune responses,and SAR regulated more DEGs than AR.Additionally,SAR exerted more extensive regulatory effects on the targets than AR.Conclusion:Salt processing mainly changes the content of chemical compounds in AR,which may indirectly optimize the proportion of the main compounds to enhance the therapeutic effect while reducing the toxicity.AR and SAR mainly ameliorate the edema due to kidney Yin deficiency by reducing inflammation and improving immunity.Finally,SAR regulates more genes and signaling pathways and exerts more extensive regulatory effects than AR.

关 键 词：Alismatis Rhizoma(AR) Salt-processed Alismatis Rhizome(SAR) Ultra-high performance liquid chromatographyquadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) TERPENOID TRANSCRIPTOMICS 

分 类 号：R285[医药卫生—中药学]