HPLC法同时测定枸杞中3种酚酸和3种黄酮化合物的含量  

Quantitative Determination of Three Phenolic Acids and Three Flavonoids of Lycium barbarum L.by HPLC

作　　者：刘淑瑛 臧娅妮 马玲 姜文霞 马宗卫 马玉霞 马小荣 LIU Shuying;ZANG Yani;MA Ling;JIANG Wenxia;MA Zongwei;MA Yuxia;MA Xiaorong(Ningxia Institute of Drug Testing,Yinchuan 750000,China)

机构地区：[1]宁夏药品检验研究院,宁夏银川750000

出　　处：《现代食品》2024年第21期224-228,共5页Modern Food

基　　金：宁夏回族自治区重点研发项目(2022BEG03174)。

摘　　要：目的:建立高效液相色谱法同时测定枸杞中3种酚酸和3种黄酮化合物含量的方法。方法:采用C18柱,以乙腈-0.5%醋酸溶液为流动相,梯度洗脱,检测器为紫外检测器,流速为1.0 mL·min^(-1),柱温为25℃,检测波长为320 nm,进样量为10μL。结果:6种化合物在各自的线性范围内具有良好的线性关系,相对标准偏差均小于5%,回收率为99.7%~106.9%。结论:本方法快速、简便、准确和重复性好,适用于检测枸杞中黄酮类和酚酸类化合物的含量。Objective:To establish a method for simultaneous determination of 3 phenolic acids and 3 flavonoids in Lycium barbarum L.by HPLC.Method:C18 column was used,acetonitrile-0.5%acetic acid solution was used as mobile phase,gradient elution,UV detector,flow rate was 1.0 mL·min_(-1),column temperature was 25℃,detection wavelength was 320 nm,sample size was 10μL.Result:The 6 compounds had a good linear relationship in their linear range,the relative standard deviations were less than 5%,and the recoveries were 99.7%to 106.9%.Conclusion:The method is rapid,simple,accurate and reproductively suitable for the determination of flavonoids and phenolic acids in Lycium barbarum L..

关 键 词：高效液相色谱法 枸杞 黄酮 

分 类 号：TS207.3[轻工技术与工程—食品科学]