宫颈癌中肽脱甲酰基酶潜在生物学机制的研究  

Transcriptomic study on the potential biological mechanism of peptide deacetylase in cervical cancer

作　　者：张璐[1] 柳毅平 郝崔培 李玢[1] ZHANG Lu;LIU Yiping;HAO Cuipei;LI Bin(Department of Gynecology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]陕西省人民医院妇科,西安710068

出　　处：《临床肿瘤学杂志》2024年第11期1041-1046,共6页Chinese Clinical Oncology

基　　金：陕西省自然科学基础研究计划资助项目(2023-JC-YB-655);陕西省人民医院科技发展孵化基金资助项目(2023YJY-11);陕西省人民医院科技人才支持计划拔尖人才资助项目(2022BJ-01)。

摘　　要：目的通过TCGA数据库研究肽脱甲酰基酶(PDF)在宫颈癌中表达及其与预后的关系,探索PDF在宫颈癌中潜在生物学机制。方法从TCGA数据库中下载宫颈癌样本的转录组数据及临床数据。分别对数据进行PDF差异分析、生存分析、独立预后分析及与临床病理参数的相关性分析。利用TIMER 2.0对PDF进行泛癌表达水平分析。采用共表达分析及GSEA富集分析鉴定PDF在宫颈癌中的相关基因、通路及细胞功能。收集2023年1月至2023年10月间在陕西省人民医院行手术治疗的50例宫颈癌患者新鲜癌组织及癌旁组织,提取组织RNA。实时荧光定量PCR(qPCR)法检测组织中PDF和RAB5IF表达情况。结果TCGA数据库显示宫颈癌中PDF高表达。生存分析结果显示,PDF低表达患者的生存时间明显长于高表达组(P<0.001)。受试者工作特征曲线结果显示,PDF表达预测宫颈癌患者1、3、5年总生存(OS)率的曲线下面积分别为0.681、0.668、0.698。PDF表达与宫颈癌肿瘤分级和浸润程度有关(P<0.05)。单因素和多因素Cox风险比例回归模型分析显示,PDF是影响宫颈癌OS的独立因素(P<0.001),风险比分别为2.555(95%CI:1.601~4.076)和2.622(95%CI:1.551~4.433)。Pearson相关性分析显示,RAB5IF与PDF的相关性最显著(r=0.8,P<0.001)。PDF和RAB5IF在50例宫颈癌组织中的表达量均高于癌旁组织(P<0.05)。Pearson相关性分析证明PDF与RAB5IF呈正有关(r=0.64,P<0.001)。结论宫颈癌中PDF高表达且与较差的预后相关,提示PDF可能作为宫颈癌新的肿瘤标志物及靶点。Objective To investigate the expression of peptide deacetylase(PDF)in cervical cancer and its correlation with prognosis through the TCGA database,and to explore its potential biological mechanisms in cervical cancer.Methods The transcriptome data and clinical data of cervical cancer samples from the TCGA database was downloaded.PDF difference analysis,survival analysis,independent prognosis analysis and correlation analysis with clinicopathological parameters were performed.The expression level of pan cancer in PDF was analyzed by TIMER 2.0.Co-expression analysis and GSEA enrichment analysis were used to identify the genes,pathways and cell functions associated with PDF in cervical cancer.Fresh cancer tissues and adjacent tissues of 50 patients with cervical cancer who underwent surgical treatment in Shaanxi Provincial People’s Hospital from January 2023 to October 2023 were collected and tissue RNA was extracted.Real-time fluorescence quantitative PCR(qPCR)was used to detect the expression of PDF and RAB5IF in tissues.Results TCGA database showed high expression of PDF in cervical cancer.Survival analysis showed that the survival time of patients with low PDF expression was significantly longer than that of those with high PDF expression(P<0.001).The results of the subject working characteristic curve showed that the area under the curve of PDF expression predicting the 1,3 and 5 year overall survival(OS)rate of cervical cancer patients was 0.681,0.668 and 0.698,respectively.The expression of PDF was correlated with the grade and invasion degree of cervical cancer(P<0.05).Univariate and multivariate Cox proportional regression analysis showed that PDF was an independent prognostic factor for cervical cancer OS(P<0.001),with hazard ratios of 2.555(95%CI:1.601-4.076)and 2.622(95%CI:1.551-4.433),respectively.Pearson correlation analysis showed that RAB5IF had the most significant correlation with PDF(r=0.8,P<0.001).The expression levels of PDF and RAB5I in 50 cases of cervical cancer were higher than those in ad

关 键 词：宫颈癌 肽脱甲酰基酶 TCGA数据库 预后 

分 类 号：R737.3[医药卫生—肿瘤]