海风藤总黄酮提取工艺及抗氧化活性研究  

Research on the Extraction Process and Antioxidant Activity of Total Flavonoids From Sea Breeze Vine

作　　者：吕双双 马志欢 张洪 廉伟伟 刘金海 LV Shuangshuang;MA Zhihuan;ZHANG Hong;LIAN Weiwei;LIU Jinhai

机构地区：[1]郑州工业应用技术学院药学与化学工程学院,河南郑州451150

出　　处：《化工设计通讯》2024年第12期159-161,共3页Chemical Engineering Design Communications

基　　金：河南省医学教育研究项目(WJLX2024193);2023年郑州工业应用技术学院教育教学改革研究与实践项目(JG-230213)。

摘　　要：利用正交实验,探究海风藤总黄酮提取方法的最佳提取工艺及从海风藤中提取出的总黄酮所具有的抗氧化活性强度。采用回流提取的方法提取海风藤中的总黄酮,基于单因素实验分析并利用正交实验来优化海风藤中的总黄酮提取工艺。采用DPPH法测定海风藤黄酮提取物的抗氧化活性。确定浓度为75%乙醇、提取温度75℃,料液比1:20,提取时间1.5 h为海风藤总黄酮的最佳提取工艺。并在此条件下提取海风藤的总黄酮测定其抗氧化活性,随着海风藤总黄酮浓度的增加,其对自由基的清除能力也在逐渐增强,在其浓度为0.229 mg/mL时的最高清除率为62.44%。该提取工艺提取效率良好,并且从海风藤中提取出的黄酮类化合物具有较为良好的抗氧化活性。Objective:to explore the optimal extraction process of total flavonoids from piper kadsura(choisy)ohwi and the antioxidant activity intensity of the total flavonoids extracted from piper kadsura(choisy)ohwi using orthogonal experiments.method:The total flavonoids in piper kadsura(choisy)ohwi were extracted using reflux extraction method,and the extraction process of total flavonoids in piper kadsura(choisy)ohwi was optimized based on single factor experimental analysis and orthogonal experiment.the antioxidant activity of sea cucumber flavonoids extract was studied by using DPPH method and total reducing power determination method.result:the optimal extraction process for total flavonoids from piper kadsura(choisy)ohwi was determined to be 75%ethanol concentration,extraction temperature of 75℃,solid-liquid ratio of 1:20,and extraction time of 1.5 hours.And under these conditions,the total flavonoids of piper kadsura(choisy)ohwi were extracted to determine its antioxidant activity.as the concentration of total flavonoids in piper kadsura(choisy)ohwi increased,its ability to scavenge free radicals gradually strengthened,with the highest clearance rate of 62.44%at a concentration of 0.229 mg/mL.using vitamin c as a reference substance,the reducing power of total flavonoids extracted from piper kadsura(choisy)ohwi at the same concentration is slightly lower than that of vitamin c.conclusion:the extraction process has good extraction efficiency,and the flavonoids extracted from piper kadsura(choisy)ohwi have good antioxidant activity.

关 键 词：海风藤 总黄酮 回流提取 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]