棉花黄萎病高效拮抗细菌的筛选及其防治效果  

Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt

作　　者：张德政 聂太礼 杨军 柯兴盛 闫得朋 聂樟清 钟敏 ZHANG De-zheng;NIE Tai-li;YANG Jun;KE Xing-sheng;YAN De-peng;NIE Zhang-qing;ZHONG Min(Cash Crops Research Institute of Jiangxi Province,Nanchang 330000,China;Jiangxi Jiangmian Technology Development Co.,Ltd.,Jiujiang 332000,Jiangxi,China;Jiangxi Agricultural Technology Promotion Center,Nanchang 330000,China)

机构地区：[1]江西省经济作物研究所,南昌330000 [2]江西省江棉科技开发有限公司,江西九江332000 [3]江西省农业技术推广中心,南昌330000

出　　处：《湖北农业科学》2024年第12期67-72,共6页Hubei Agricultural Sciences

基　　金：江西省棉花产业技术体系资金项目(JXRS-22)。

摘　　要：以棉花黄萎病菌(Verticillium dahliae Kleb)为指示菌,采用对峙培养、代谢物抑菌试验筛选高活性生防菌株。通过盆栽试验明确供试菌株的防病效果。结果表明,从棉花根际土壤分离的1000余株细菌中筛选到162株具有生防效果的菌株,采用平板对峙法进行复筛,最终获得4株综合效果较好的菌株。其中菌株XJ5-6和XJ6-96的抑菌效果较好,抑菌带宽度均超过5.0 mm,抑菌率分别达67.54%和66.86%。盆栽试验结果表明,菌株XJ5-6和XJ6-96混合接种对棉花黄萎病具有较好的防治作用,其防治效果在28 d和40 d分别为32.2%和29.9%,菌株XJ6-96能提高棉花种子的萌发率。Using Verticillium dahliae Kleb as indicator bacteria,the biocontrol strains with high activity were screened by confronation culture and metabolite inhibition test.The preventive effect of the tested strain was determined by pot experiment.The results showed that 162 strains of bacteria with biocontrol effect were screened from more than 1000 strains isolated from cotton rhizosphere soil,and 4 strains with good comprehensive effect were obtained by using the plate face-off method.Strains XJ5-6 and XJ6-96 showed better in⁃hibitory effects,with the band width exceeding 5.0 mm and the inhibitory rate reaching 67.54%and 66.86%,respectively.The results of pot experiment showed that XJ5-6 and XJ6-96 combined inoculation had a good control effect on verticillium wilt of cotton,and the control effect was 32.2%and 29.9%on 28 and 40 days,respectively.XJ6-96 could improve the germination rate of cotton seed.

关 键 词：棉花黄萎病 根际细菌 高效拮抗细菌 抑菌活性 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治] S476.19[农业科学—植物保护]