枯草芽孢杆菌对沙生植物种子萌发和幼苗生长生理特征的影响  

Effects of Bacillus subtilis on the seed germination and seeding growth and physiological characteristics of psammophytes

作　　者：吴玮婷 刘振婷 高广磊[1,2,3] 孙哲 张金鑫 王天博 曹红雨 丁国栋 WU Weiting;LIU Zhenting;GAO Guanglei;SUN Zhe;ZHANG Jinxin;WANG Tianbo;CAO Hongyu;DING Guodong(School of Soil and Water Conservation,Beijing Forestry University,100083,Beijing,China;Yanchi Ecology Research Station of the Mu Us Desert,751500,Yanchi,Ningxia,China;State Key Laboratory of Efficient Production of Forest Resources,100083,Beijing,China;China Electric Power Planning&Engineering Institute,100120,Beijing,China;China Power Engineering Consulting Group Co.Ltd.,100120,Beijing,China)

机构地区：[1]北京林业大学水土保持学院,北京100083 [2]宁夏盐池毛乌素沙地生态系统国家定位观测研究站,宁夏盐池751500 [3]林木资源高效生产全国重点实验室,北京100083 [4]电力规划总院有限公司,北京100120 [5]中国电力工程顾问集团有限公司,北京100120

出　　处：《中国水土保持科学》2024年第6期70-76,共7页Science of Soil and Water Conservation

基　　金：内蒙古自治区科技计划项目“毛乌素沙地系统修复新材料、新技术集成示范”(2022YFHH0131);中国能源建设股份有限公司重大科技项目“大型新能源基地综合技本研究与应用”(CEEC2022-ZDY F-03)。

摘　　要：枯草芽孢杆菌(Bacillus subtilis)是一种新型微生物治沙材料,在防沙治沙领域应用潜力巨大,但作为一种典型的植物根际促生菌,其对沙生植物定殖和生长的影响尚不明确。以沙冬青和柠条为研究对象,研究揭示不同枯草芽孢杆菌添加量对种子萌发和幼苗生长生理特征的影响。结果表明:1)枯草芽孢杆菌可提高沙冬青和柠条种子萌发率,最适菌剂施用量分别为3和10 g/kg,其对沙冬青和柠条幼苗冠幅生长无显著影响(P>0.05),仅在10 g/kg处理组显著促进柠条幼苗株高生长(P<0.05);2)枯草芽孢杆菌可显著增加沙冬青幼苗叶绿素含量(P<0.05),显著降低沙冬青和柠条幼苗丙二醛和脯氨酸含量(P<0.05),且最适菌剂施用量均为5 g/kg。枯草芽孢杆菌可改善沙生植物生理生化特征,进而促进幼苗生长,但植物生长响应存在滞后性;不同枯草芽孢杆菌菌剂施用量对植物影响存在差异,且对沙冬青影响弱于柠条。研究结果可为丰富和完善枯草芽孢杆菌防沙治沙技术研发提供理论参考。[Background]Bacillus subtilis is an emerging microbial material which potentially contribute to desertification combating.However,as a typical plant growth-promoting rhizobacteria,its effects on the plant colonization and growth in a desert environment still remain unclear.This study is aimed to investigate the effect of B.subtilis on the physiological characteristics of seed germination and seeding growth of psammophyte.[Methods]The controlled experiments were conducted to determine the seed germination,seeding growth and physiological characteristics of Ammopiptanthus mongolicus and Caragana korshinskii using different bacterial application amount(0,1,3,5,7 and 10 g/kg).The changes of seed germination and the physiological characteristics of seeding growth were investigated,including contents of chlorophyll,malondialdehyde and proline.[Results]1)B.subtilis increased the seed germination rate of A.mongolicus and C.korshinskii,and the optimal dosage were 3 and 10 g/kg,respectively.Meanwhile,it had no significant effect on the seedling crown(P>0.05),only 10 g/kg dosage significantly impacted the height of C.korshinskii seedings(P<0.05).2)B.subtilis significantly affected the physiological and biochemical characteristics of A.mongolicus and C.korshinskii(P<0.05).The chlorophyll content of A.mongolicus significantly increased(P<0.05)and the malondialdehyde and proline of A.mongolicus and C.korshinskii significantly decreased(P<0.05),and the optimal dosage was 5 g/kg.[Conclusions]B.subtilis improves the plant physiological and biochemical characteristics therefore promotes the seeding growth,but this effect is limited with a lagging performance.Moreover,it varies with the bacterial application amount and plant species,the C.korshinskii is more impressionable than A.mongolicus.This improved information will provide theoretical basis and scientific support for desertification combating technology exploration using B.subtilis.

关 键 词：枯草芽孢杆菌 沙冬青 柠条 种子萌发 幼苗生长生理 

分 类 号：S775[农业科学—森林工程]