黄山毛峰茶多酚提取工艺优化及体外抗菌活性研究  

Optimization of Extraction Process and in vitro Antimicrobial Activity of Huangshan Maofeng Tea Polyphenols

作　　者：赵东贤[1] ZHAO Dongxian(College of Medicine,Huainan United University,Huainan 232038)

机构地区：[1]淮南联合大学医学院,淮南232038

出　　处：《吉林农业科技学院学报》2024年第6期1-4,9,共5页Journal of Jilin Agricultural Science and Technology University

基　　金：安徽省高校自然科学研究重点项目(2022AH052931)。

摘　　要：以黄山毛峰为试材,采用超声波辅助乙醇法提取黄山毛峰茶多酚,在单因素试验基础上利用正交试验优化提取工艺,同时结合药敏试验评估茶多酚对志贺杆菌的抗菌活性。结果表明:黄山毛峰茶多酚最佳提取工艺为乙醇质量浓度为55%,料液比为1∶35 g·mL^(-1),超声次数为130次,浸提温度为65℃。在此条件下黄山毛峰茶多酚提取率为17.22%,药敏试验显示最佳条件下所得的茶多酚提取液对志贺杆菌的抑菌圈直径为9.6±0.8 mm。该研究为茶多酚提取工艺和其抗菌活性分析提供参考依据。Huangshan Maofeng tea was used as the raw material,and tea polyphenols were extracted using an ultrasound-assisted ethanol extraction method.Based on single-factor experiments,the extraction process was optimized through orthogonal experiments,and the antimicrobial activity of the tea polyphenols against Shigella was evaluated using antimicrobial susceptibility tests.The results showed that the optimal extraction conditions for Huangshan Maofeng tea polyphenols were 55%ethanol mass concentration,solid-liquid ratio of 1∶35 g·mL^(-1),130 ultrasound cycles,and an extraction temperature of 65℃.Under these conditions,the extraction yield of Huangshan Maofeng tea polyphenols was 17.22%.Antimicrobial susceptibility tests revealed that the diameter of the inhibition zone for Shigella was 9.6±0.8 mm under the optimal conditions.This study provides a reference for the extraction of tea polyphenols and their antimicrobial activity evaluation.

关 键 词：茶多酚 超声波辅助提取 志贺杆菌 正交试验设计 

分 类 号：R284.2[医药卫生—中药学]