Effect of extraction treatments on the functional properties of free and bound phenols in apple seeds  

作　　者：Yubin Ding Ksenia Morozova Lucrezia Angeli Alessandra Gasparini Giovanna Ferrentino Matteo Scampicchio 

机构地区：[1]Faculty of Agricultural,Environmental and Food Sciences,Free University of Bozen-Bolzano,Piazza Università1,39100,Bolzano,Italy

出　　处：《Food Bioscience》2023年第3期1098-1106,共9页食品生物科学（英文）

基　　金：Award Number:Project code PE00000003,Concession Decree No.1550 of October 11,2022 adopted by the Italian Ministry of University and Research,CUP D93C22000890001,Project title“ON Foods-Research and innovation network on food and nutrition Sustainability,Safety and Security-Working ON Foods”.

摘　　要：Apple seeds are one of the main by-products of the food industry.This research aims to characterize free and bound phenols from apple seeds first defatted with three methods:(1)supercritical CO_(2),(2)ultrasound assisted and(3)Soxhlet extraction.Next,defatted seeds were extracted with hydroalcoholic maceration to give free phenols fraction and a solid residue.The latter was further extracted by alkaline hydrolysis to give bound phenols fraction.The time,temperature,concentration of NaOH and solid-to-liquid ratio were optimized for maximum extraction of bound phenols.Results showed that,regardless to the defatting method used,the highest phenol content was found mainly in the bound fraction(up to 31 mg g^(-1) GAE),which was eight times higher than the free fraction.Also,the antioxidant capacity(DPPH and ORAC)of the bound fraction was twice that of the free fraction.Such differences were next linked to the polyphenol profile(HPLC-DAD),and to the electron transfer properties(HPLC-coulometric detector).Results showed that the highest electron transfer properties of bound phenols were explained with higher content and lower half-wave potential of redox species like protocatechuic acid,salvianic acid A,and caffeic acid(identified by HPLC HR-MS/MS).Instead,the redox properties of free phenol extracts were attributed mainly to phlorizin.Overall,bound phenols in defatted apple seeds are a rich source of antioxidants that can be easily extracted by alkaline hydrolysis.

关 键 词：Apple seeds Non-extractable polyphenol HPLC Coulometric array detector Food by-products 

分 类 号：TS201[轻工技术与工程—食品科学]