Immunostimulating activities and anti-cancer efficacy of rhamnogalacturonan-I rich polysaccharide purified from Panax ginseng leaf  

作　　者：Seung-U Son Hee Won Lee Kwang-Soon Shin 

机构地区：[1]Department of Food Science and Biotechnology,Kyonggi University,Suwon,16227,Republic of Korea [2]Transdisciplinary Major in Learning Health System,Department of Integrated Biomedical and Life Science,Korea University,Seoul,02841,Republic of Korea

出　　处：《Food Bioscience》2023年第3期1218-1227,共10页食品生物科学（英文）

基　　金：supported by the 2018 grant from the Korean Society of Ginseng,funded by the Korean Ginseng Corporation.

摘　　要：This study aimed to evaluate the anti-cancer effects as well as stimulation of various immune related factors such as complementary system,macrophages,and NK cells of polysaccharides isolated from Panax ginseng(P.ginseng)leaf.The polysaccharide fractions(PGEP,PGEP-I,-II,and-III)isolated from P.ginseng were contained rhamnose(Rha),arabinose(Ara),galactose(Gal),galacturonic acid(GalA),and glucuronic acid(GlcA),all of which are typical sugar components of rhamnogalacturonan(RG)-I.Only PGEP-I reacted with theβ-glucosyl Yariv reagent(37.29%),indicating the presence of type-II arabinogalactan.Additionally,methylation analysis indicated the PGEP-I mainly comprised 19 different sugar linkages including 4-linked GalpA,2,4-linked Rhap,3,6-linked Galp,5-linked Araf,terminal GlcpA,and terminal Arap which are characteristics of RG-I.Meanwhile,PGEP-I enhanced the production of cytokines such as interleukin(IL)-6,IL-12,and tumor necrosis factor-αand augmented the complement system.The intravenous administration of PGEP-I activated the natural killer(NK)cells in a dose-dependent manner against YAC-1.Prophylactic intravenous administration of PGEP-I significantly suppressed Colon26-M3.1 carcinoma inoculation-induced lung cancer(48.7%).Moreover,the anti-cancer effects of PGEP-I were significant even though NK cell functions were blocked by anti-asialo GM1.The findings suggest that PGEP-I,an RG-I polysaccharide,not only activates the complement system,macrophages and NK cells,but also has strong anticancer effects that inhibit lung cancer.

关 键 词：Immune system Panax ginseng RHAMNOGALACTURONAN-I 

分 类 号：TS201.4[轻工技术与工程—食品科学]