Screening,characterization of traceα-glucosidase inhibitors from the root of Pueraria lobata and evaluation of their hypoglycemic activity  

作　　者：Haiping Xiang Ping Xu Weiyi Wen Huiyun Qiu Chu Chu Qingsong Shao Shengqiang Tong 

机构地区：[1]College of Pharmaceutical Science,Zhejiang University of Technology,Huzhou,313200,China [2]Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,Zhejiang A&F University,Hangzhou,311300,China

出　　处：《Food Bioscience》2023年第3期1405-1411,共7页食品生物科学（英文）

基　　金：financially supported by the National Natural Science Foundation of China(21978266);Natural Science Foundation of Zhejiang Province(LBY22H280004);Academy of Science&Tech-nology of Zhejiang University of Technology(KYY-HX-20210732,KYYHX-20210066,KYY-HX-20200221,KYY-HX-20190182).

摘　　要：Pueraria lobata(Willd.)Ohwi(Gegen)is a nutritious medicine food homology plant that has been recorded in many ancient remedies for diabetes.However,its hypoglycemic active components and related mechanisms have not been fully elucidated.In this study,potentialα-glucosidase inhibitors in Pueraria lobata were investigated by liquid-liquid refined extraction,high-resolution profiling and their hypoglycemic activity were evaluated.Ten compounds showingα-glucosidase inhibitory bioactivity were separated and identified,including two new compounds,8-geranyl-7,3′,4′-trihydroxy-isoflavone(4)and 8-geranyl-7,2′,4′-trihydroxy-isoflavone(6),and eight known compounds.All screened compounds showed potential hypoglycemic activity,in which compounds 4,6,7,8,9 and 10 showed strong inhibitory activity(IC50<8μM).Furthermore,molecular docking simulation revealed binding energy and interacting amino acid residues of the screened compounds withα-glucosidase,strongly supporting their potential asα-glucosidase inhibitors.

关 键 词：Pueraria lobata Liquid-liquid refined extraction High-resolution profiling α-Glucosidase inhibition 

分 类 号：TS201[轻工技术与工程—食品科学]