Anti-oxidative and anti-inflammatory effects of different lutein isomers in Caco-2 cell model  

作　　者：Ning Cui Cheng Yang Xuemei Chen Jian Zhang Lianfu Zhang 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China [2]College of Food,Shihezi University,Beisi Road,Shihezi,Xinjiang,832003,PR China

出　　处：《Food Bioscience》2023年第3期1523-1531,共9页食品生物科学（英文）

基　　金：funded by the Natural Science Foundation of China(31901654);the Natural Science Foundation of Jiangsu Province(BK20190592).

摘　　要：The purpose of this work was to investigate the differences in the anti-oxidative stress and anti-inflammatory effects between all-E(trans)-lutein and its 9Z(cis)-and 13Z-isomers using the human intestinal Caco-2 cell model.I-TiO_(2) catalyst was used to isomerize all-E-lutein to obtain 9Z-lutein and 13Z-lutein.In anti-oxidative stress studies,all three lutein isoforms could significantly reduce H2O_(2)-induced interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)secretion(p<0.05)by 50%or more at 4μM.At lower concentration(2μM),9Z-lutein and 13Z-lutein showed higher protection against oxidative stress than all-E-lutein by significantly reducing IL-8 secretion by 48%and 40%(p<0.05),respectively.In addition,three lutein isomers,especially 9Z-lutein could up-regulate the activity of antioxidant enzymes including catalase(CAT),glutathione peroxidase(GPx),gluta-thione reductase(GR),and the content of endogenous antioxidant glutathione(GSH)at 2μM.In the anti-inflammatory assay,9Z-lutein and all-E-lutein exhibited higher anti-inflammatory effects than 13Z-lutein in reducing the secretion and expression of IL-8 in TNF-αinduced inflammatory Caco-2 cells.Compared with the positive control,9Z-lutein and all-E-lutein could reduce the expression of the IL-8 gene by 49%and 52%,respectively at 4μM.This study provided a theoretical basis for the potential health benefits of Z-luteins.

关 键 词：All-E-lutein 9Z-lutein 13Z-lutein Anti-oxidative stress ANTI-INFLAMMATORY Caco-2 cells 

分 类 号：TS201[轻工技术与工程—食品科学]