High Fischer ratio oligopeptides from hard-shelled mussel:Preparation and hepatoprotective effect against acetaminophen-induced liver injury in mice  

作　　者：Shuo-Lei Zheng Yue-Zheng Wang Yu-Qin Zhao Chang-Feng Chi Wang-Yu Zhu Bin Wang 

机构地区：[1]Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products,School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan,316022,China [2]National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources,National Engineering Research Center of Marine Facilities Aquaculture,School of Marine Science and Technology,Zhejiang Ocean University,Zhoushan,316022,China [3]Cell and Molecular Biology Laboratory,Zhoushan Hospital,Zhejiang Province,Zhoushan,316021,China

出　　处：《Food Bioscience》2023年第3期1551-1564,共14页食品生物科学（英文）

基　　金：funded by the National Natural Science Foundation of China,China(No.82073764);the Ten-thousand Talents Plan of Zhe-jiang Province,China(No.2019R52026).

摘　　要：High Fischer ratio oligopeptides(HFOPs)have a variety of physiological activities,but there are few literatures reporting the hepatoprotective effects of HFOPs against acetaminophen(APAP)-induced liver injury.The ob-jectives of this study were to establish the preparation process of HFOPs from hard-shelled mussel(Mytilus coruscus)(HFOPs-HM)and investigate the hepatoprotective effects and potential mechanisms of HFOPs-HM against APAP-induced liver injury in mice.Therefore,trypsin and papain were selected as the endo and exog-enous proteases for preparing HFOPs-HM according to the degree of hydrolysis(DH)of hard-shelled mussel hydrolysates,and activated carbon of XHJ-200 displayed the best effect to remove aromatic amino acids ac-cording to the Fischer ratio(OD220/OD280)and its parameters were optimized as adsorption time of 3.0 h and solid-liquid ratio of 1:10.The HFOPs-HM’s Fischer ratio and average molecular weight were 26.03(>20)and 878.54 Da,respectively.Furthermore,a mice model of liver injury induced by APAP was established.HFOPs-HM could significantly improve liver index and histopathological features,decrease the serum alanine aminotrans-ferase(ALT)and aspartate transaminase(AST)activities,increase the glutathione content and superoxide dis-mutase(SOD)and catalase(CAT)activities.Further research demonstrated that HFOPs-HM could alleviate liver oxidative stress in liver tissue via activating the Nrf2 pathway to initiate the intracellular antioxidant enzyme system.In addition,HFOPs-HM also suppressed liver inflammation by downregulating the secretion of TNF-α,IL-1β,and IL-6.Thus,HFOPs-HM could serve as an auxiliary functional dietary molecule applied in function products to alleviate drug-induced liver injury.

关 键 词：Hard-shelled mussel(Mytilus coruscus) High Fischer ratio oligopeptides(HFOPs) Hepatoprotective effect Acetaminophen(APAP)-Induced liver injury Oxidative stress Inflammatory 

分 类 号：TS201.2[轻工技术与工程—食品科学]