Investigating the effect of extraction procedure on the anti-inflammatory metabolites of olibanum resin from different Boswellia species through LC-MS/MS-based metabolomics  

作　　者：Hanaa M.M.Elhaddad Hala M.Hammoda Doaa A.Ghareeb Fatma A.A.Mahmoud Ahmed Hussein Mokhtar Ibrahim Yousef Reham S.Darwish Eman Shawky 

机构地区：[1]Department of Pharmacognosy,Faculty of Pharmacy,Alexandria University,Egypt [2]Bio-screening and Preclinical Trial Lab,Biochemistry Department,Faculty of Science,Alexandria University,Alexandria,Egypt [3]Biochemistry Department,Faculty of Science,Alexandria University,Alexandria,Egypt [4]Department of Biotechnology,Institute of Graduate Studies and Research,Alexandria,Egypt [5]Department of Environmental Studies,Institute of Graduate Studies and Research,Alexandria,Egypt

出　　处：《Food Bioscience》2023年第3期1665-1677,共13页食品生物科学（英文）

摘　　要：Olibanum is a resinous extract extracted from several species of Boswellia trees.This works aims to contribute to the research on the chemistry and anti-inflammatory activity of raw,acidic and neutral fractions of different olibanum species to determine the effects of different extraction procedures on the composition of anti-inflammatory constituents.Chemical profiling of different fractions of Boswellia species was attempted using UPLC/MS/MS coupled to chemometric analysis.The anti-inflammatory activity of the tested extracts was assessed on LPS-stimulated WBCs through four pro-inflammatory cytokines(TNF-α,IL-6,IL-1βand IFN-γ)in addition to reveal the efficacy-directed markers using multivariate analysis.UPLC/MS/MS analysis led to the annotation of 77 compounds belonging to several chemical classes.Chemometric models indicated that samples segregation was based on the geographical source of the oleo-gum-resin rather than extraction procedure.Acidic and neutral extracts of B.carterii and B.sacra alcoholic extract were strongly correlated to inhibition of the pro-inflammatory markers IL-1βand TNF-αwhile there was a strong correlation between the raw extract of B.frereana and the alcoholic extract of B.serrata activity to IFN-γinhibition.Meanwhile,the most active extracts against IL-6 were the acidic and raw extracts of B.serrata and B.frereana.

关 键 词：Frankincense EXTRACTION ANTI-INFLAMMATORY Metabolomics UPLC/MS/MS 

分 类 号：TS201[轻工技术与工程—食品科学]