Accelerated solvent extraction of red onion peel extract and its antimicrobial,antibiofilm,and quorum-sensing inhibition activities against Listeria monocytogenes and Chromobacterium violaceum  

作　　者：Nitin Dhowlaghar Tushar Dhanani Syamkumar Sivasankara Pillai Bhimanagouda S.Patil 

机构地区：[1]Vegetable and Fruit Improvement Center,Texas A&M University,USDA National Center of Excellence for Melon,1500 Research Parkway,Suite A120,College Station,TX,77845,USA

出　　处：《Food Bioscience》2023年第3期1826-1833,共8页食品生物科学（英文）

基　　金：supported by the USDA-NIFA-SCRI-2017-51181-26834 through the National Center of Excellence for Melon at the Vegetable and Fruit Improvement Center of Texas A&M University,College Station,Texas and also Institute of Advancing Health through Agriculture.

摘　　要：In this study,we used accelerated solvent extraction(ASE)to extract flavonoids and anthocyanins from dried red onion peels,producing red onion peel extract(ROPE).High-performance liquid chromatography analysis of ROPE obtained using ASE identified important flavonols and anthocyanins.Total flavonoid and anthocyanin contents measured from ROPE were 459.15 mg/g±16.45(DW)and 33.14 mg/g±0.99(DW),respectively.ROPE antimicrobial activity against L.monocytogenes showed strain-specific effects,where the minimum inhibitory concentration(MIC)for V7 was two-fold greater than for EGD and Scott A(i.e.,25.82 vs.12.91 mg quercetin-3,4′-diglucoside equivalents/gram[mg QdGE/g]ROPE).ROPE significantly inhibited(P<0.05)biofilm formation by all three strains at sub-inhibitory concentrations(0.40-6.45 mg QdGE/g)in a concentration-dependent manner.Pre-formed biofilms of mixed L.monocytogenes strains on stainless steel were inactivated after 48 h at 22℃ only with higher concentrations of ROPE(25.82-56.63 mg QdGE/g).Sub-inhibitory concentrations of ROPE also inhibited L.monocytogenes motility and suppressed violacein pigment production in Chromobacterium violaceum ATCC 12472,suggesting that ROPE inhibited quorum sensing.Therefore,our data indicate that red onion peel waste has potential as a source of natural antibacterial compounds as an approach to food safety in the circular economy.

关 键 词：Listeria monocytogenes Red onion peel Biofilm inhibition Subinhibitory concentrations Accelerated solvent extraction Quorum sensing Chromobacterium violaceum 

分 类 号：TS201[轻工技术与工程—食品科学]