Characteristics and functions of Limosilactobacillus reuteri(Lactobacillus reuteri)from the intestine of mice supplemented with three dietary components based on genome resequencing and metabolite analysis  

作　　者：Ziyan Wang Ying Feng Jiaojiao Han Chenyang Lu Jun Zhou Zhonghua Wang Xiurong Su 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo,China [2]School of Marine Science,Ningbo University,Ningbo,China [3]Key Laboratory of Aquaculture Biotechnology(Ningbo University)Ministry of Education,Ningbo University,Ningbo,China [4]College of Life Sciences,Tonghua Normal University,Tonghua,China [5]Shandong Beiyou Biotechnology Co.,Ltd,Weifang,China

出　　处：《Food Bioscience》2023年第3期1983-1992,共10页食品生物科学（英文）

基　　金：sponsored by the Natural Science Foundation of Zhejiang Province(LY19C010003);the Natural Science Foundation of Zhejiang Province(LQ22D060002);the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(ZS20190105);the K.C.Wong Magna Fund of Ningbo University.

摘　　要：Limosilactobacillus reuteri is a resident probiotic in the intestines of animals with strong environmental adaptability.In the current study,to elucidate the adaptation strategies of L.reuteri to the intestine of mice supplemented with three dietary components(oil,peptide and cellulose),whole genome resequencing and determination of intracellular metabolites were carried out.The resequencing results showed that L.reuteri TO60(LR-TO)from the intestines of mice supplemented with high docosahexaenoic acid(DHA)tuna oil,or fermented Benincasa hispida culture(L.reuteri FB151,abbreviated as LR-FB)had relatively consistent single nucleotide polymorphisms(SNPs)and insertions and deletions(InDels),and those of L.reuteri from the intestines of mice supplemented with Rhopilema esculentum polypeptides(L.reuteri RE225,abbreviated as LR-RE)were quite different from them.The KEGG functional annotation of resequencing and the intracellular metabolites suggested that in all of the L.reuteri adapted to the intestinal environment mainly by regulating carbohydrate metabolism and membrane transport metabolic pathways.However,there were still some differences in their genes and metabolites,which may be related to the changes in the intestinal environment caused by three different types of dietary interventions.Our study analyzed the adaptation mechanism of L.reuteri to different isolated sources at the genetic level and metabolic level,which is helpful to clarify the adaptation strategies of probiotics to the intestinal environment,and provide supplementary information for the use of dietary nutritional supplements.

关 键 词：Limosilactobacillus reuteri Whole genome resequencing Intracellular metabolites High DHA tuna Oil Fermented Benincasa hispida culture Rhopilema esculentum polypeptides 

分 类 号：TS201[轻工技术与工程—食品科学]