Simple isocratic RP-UHPLC-MS methods for the determination of steviols and its glycosides:Quality control perspectives  

作　　者：Pawan Kumar Dinesh Kumar Vivek Yadav Sushil K.Maurya Rajiv K.Tonk Dinesh Kumar 

机构地区：[1]Department of Pharmaceutical Chemistry,School of Pharmaceutical Science,Delhi Pharmaceutical Sciences&Research University,India [2]Chemical Technology Division,CSIR-Institute of Himalayan Bioresource Technology,Palampur,176061,HP,India [3]Department of Chemistry,Faculty of Science,University of Lucknow,Lucknow,226007,UP,India

出　　处：《Food Bioscience》2023年第3期1999-2012,共14页食品生物科学（英文）

摘　　要：Stevia is well-known for its unique non-caloric intense sweetness and recognising as one of the most acceptable sweeteners.Therefore,quality control and metabolite profiling perspectives,two isocratic reverse-phase UPLC-PDA-ELSD-MS methods were developed to determine nine diterpene glycosides(method A)and isomeric compounds(steviol and isosteviol;method B).Both the methods were validated and found as per the ICH guidelines for accuracy(recovery),precision(Intraday,interday,and intermediate precision),linearity,and regression coefficient(r2),ruggedness(eluent concentration±2 mM,column oven temperature±3℃ and retention time),and system suitability(theoretical plate count,asymmetric factor,injection precision and resolution).LOD for steviol and its glycosides were found between 0.92 and 5.22μg/mL while LOQ were 2.78-18.29μg/mL,respectively.Validated methods had shown only the presence of steviol glycosides in Stevia rebaudina samples while LC-ESI-MS/MS revealed the identification of 46 different class of molecules.These methods will be helpful to monitor quality in Stevia production and its derived products.

关 键 词：Stevia rebaudina Steviols Steviol glycosides Quality control Diterpene glycosides UHPLC-MS/MS 

分 类 号：TS201[轻工技术与工程—食品科学]