The immunomodulatory activity of levan in RAW264.7 macrophage varies with its molecular weights  

作　　者：Pornpun Aramsangtienchai Warisara Raksachue Sirintip Pechroj Klaokwan Srisook 

机构地区：[1]Department of Biochemistry and Research Unit of Natural Bioactive Compounds for Healthcare Products Development,Faculty of Science,Burapha University,Chonburi,20131,Thailand [2]Center of Excellence for Innovation in Chemistry,Burapha University,20131,Thailand

出　　处：《Food Bioscience》2023年第3期2023-2029,共7页食品生物科学（英文）

基　　金：financially supported by the Research and Development Fund,Burapha University(Grant no.12/2562&10/2565);the Science Innovation Facility,Faculty of Science,Burapha University(SIF-IN-60300003);the Center of Excellence for Innovation in Chemistry(PERCH-CIC);the Commission on Higher Education,Ministry of Education,Thailand;the Scientific Instruments Center,School of Science,King Mongkut’s Institute of Technology Ladkrabang.

摘　　要：Levan is a fructose homopolymer obtained from both plants and microorganisms.Microbial levan is typically produced in a variety of molecular weights(MW).Previous studies have shown that different MW of levan exhibit different biological activities.Though levan was reported for its immunomodulatory effect by increasing nitric oxide(NO)production in RAW264.7 macrophage cells,whether its MW can affect this activity remains unknown.In this study,the effect of different levan MW from Bacillus subtilis on its immunoregulatory properties was investigated.B.subtilis was cultivated using various cultivation parameters to obtain levan of different MW.Three weight average MW of levan,>8×10^(5) Da(M1),1.5×10^(4) Da(M2)and 3.7×10^(3) Da(M3),were selected to test for their effect on modulating NO levels in RAW264.7 cells.Our results showed that M2 increased NO production in a dose-dependent manner,and more effectively than that of M1 and M3.Consistent with these findings,M2 significantly stimulated inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX2)protein expression,while less pronounced effects were observed in the cells treated with M1 and M3.Therefore,our results demonstrate that various MW of levan have varying effects on its immunoregulatory activities in RAW264.7 cells.Furthermore,our findings suggest that the size of levan should be taken into account when employing levan as one of the active ingredients in functional foods and immunomodulatory products.

关 键 词：LEVAN Molecular weights Immunomodulatory activities 

分 类 号：TS201[轻工技术与工程—食品科学]