Culture-independent assessment of probiotic supplement consistency in commercially available probiotic supplements  

作　　者：Aycan Gundogdu O.Ufuk Nalbantoglu Ilknur Sarikaya Gizem Karis Meryem N.Erdogan Mehmet Hora Varol Tunali 

机构地区：[1]Department of Microbiology and Clinical Microbiology,Faculty of Medicine,Erciyes University,Kayseri,Turkey [2]Genome and Stem Cell Center,Erciyes University,Kayseri,Turkey [3]Department of Computer Engineering,Erciyes University,Kayseri,Turkey [4]Department of Bioinformatics and Systems Biology,Institute of Health Sciences,Erciyes University,Kayseri,Turkey [5]Department of Molecular Biology and Genetics,Gevher Nesibe Genome and Stem Cell Institute,Erciyes University,Kayseri,Turkey [6]Medical Parasitology Department,Faculty of Medicine,Celal Bayar University,Manisa,Turkey [7]Emergency Medicine Department,Izmir Metropolitan Municipality Esrefpasa Hospital,Izmir,Turkey

出　　处：《Food Bioscience》2023年第3期2118-2125,共8页食品生物科学（英文）

摘　　要：The probiotic products market has experienced significant growth in recent years,driven by increasing consumer demand for health benefits.This demand is largely fueled by the widespread promotion of probiotic products,and is compounded by relatively lax regulations regarding these products compared to those for drugs.In this study,we sought to evaluate the consistency of product labels and contents for probiotic supplements in the Turkish and USA markets.A total of 44 commercially available probiotic products were included in the analysis,comprising 40 products from Turkey and 4 from the USA.Shotgun metagenome sequencing was performed to determine the microbiological content and functional characteristics of the products.Additionally,we evaluated the presence of antibiotic-resistance genes and virulence genes in each product.Our findings showed that 38.6%of the products contained microorganisms not indicated on their labels,while 15.9%of products did not contain one or more of the probiotic microorganisms specified on their labels.Moreover,over half of the probiotic products(68.2%)were found to contain at least one resistance gene,and 40.9%of the products carried multiple virulence genes.Streptococcus thermophilus being the primary contributor to the detected virulence genes.This study is the first to examine the label and content discrepancies of probiotic products in the Turkish market and one of the largest to analyze resistance gene profiles of probiotic supplements in the literature.These findings may suggest a potential public health risk and the need for whole genome sequencing analysis of probiotic supplements.

关 键 词：Probiotic products Shotgun metagenome sequencing Antibiotic-resistance genes Label discrepancies 

分 类 号：TS201.3[轻工技术与工程—食品科学]