Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme from Bacillus thuringiensis PL14  

作　　者：Ahlem Eddehech Renaud Rahier Danilo Donnarumma Francesca Rigano Alexandre Noiriel Abdelkarim Abousalham Francesco Cacciola Luigi Mondello Zied Zarai 

机构地区：[1]University of Sfax,ENIS,Laboratory of Biochemistry and Enzymatic Engineering of Lipases,Sfax,Tunisia [2]Univ Lyon,UniversitéLyon 1,Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires(ICBMS),UMR 5246 CNRS,Génie Enzymatique,Membranes Biomimétiques et Assemblages Supramoléculaires(GEMBAS),Bât Raulin,43 Bd du 11 Novembre 1918,F-69622,Villeurbanne Cedex,France [3]Department of Chemical,Biological,Pharmaceutical and Environmental Sciences,University of Messina,Messina,Italy [4]Department of Biomedical,Dental,Morphological and Functional Imaging Sciences,University of Messina,Messina,Italy [5]Chromaleont s.r.l.,c/o,Department of Chemical,Biological,Pharmaceutical and Environmental Sciences,University of Messina,Messina,Italy [6]Department of Sciences and Technologies for Human and Environment,University Campus Bio-Medico of Rome,Rome,Italy

出　　处：《Food Bioscience》2023年第3期3029-3037,共9页食品生物科学（英文）

基　　金：supported by“Ministry of Higher Education Scientific research Tunisia”;Shimadzu Corporation;Merck Life Science for their continuous support.

摘　　要：The present work reports the heterologous expression and biochemical characterization of a novel phosphati-dylinositol phospholipase C from Bacillus thuringiensis PL14(PI-PLCBt)with potential for oil degumming.As degumming is a necessary refining step for all crude vegetable oils,enzymatic degumming tests were performed on crude soybean oil using rPI-PLCBt.Its efficiency was investigated by determining the phosphorus and diac-ylglycerol(DAG)contents after different types of treatment.Although the water and chemical degumming process can efficiently reduce the phosphorus content,a specific enzymatic process can still be selected giving its high degumming efficiency of 89.4%,with a noticeable reduction in phosphorus content to 13.7 mg kg^(-1),close to the values of the chemical approach,and a high increase in DAG.Accordingly,enzymatic degumming could be adopted as an alternative to traditional degumming making the process more efficient,eco-friendly and,at the same time,increasing the nutritional value of the final product.

关 键 词：Bacillus thuringiensis Phosphatidylinositol phospholipase C Heterologous expression Enzymatic degumming Diacylglycerols content Phosphorus content 

分 类 号：TS201[轻工技术与工程—食品科学]