植物乳杆菌和罗伊氏乳杆菌对哺乳仔猪生长性能、肠道功能和盲肠菌群的影响  

Effects of Lactobacillus plantarum and Lactobacillus reuteri on Growth Performance,Intestinal Function and Cecal Microbiota of Suckling Piglets

作　　者：王琪[1] 肖融[1] 齐仁立[1] 王敬[1] 邱小宇 黄金秀[1] WANG Qi;XIAO Rong;QI Renli;WANG Jing;QIU Xiaoyu;HUANG Jinxiu(Center of Technology Innovation for Pig(Chongqing),Chongqing Academy of Animal Sciences,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,生猪技术创新中心(重庆),重庆402460

出　　处：《动物营养学报》2024年第12期7658-7668,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX0378);重庆市技术创新与应用发展项目(21210);国家生猪技术创新中心先导科技项目(NCTIP-XD/B04)。

摘　　要：本试验旨在研究植物乳杆菌和罗伊氏乳杆菌对哺乳仔猪生长性能、肠道功能和盲肠菌群的影响。选取12窝(每窝8头仔猪)健康的1日龄“长白×荣昌”哺乳仔猪(母猪胎次和预产期相近),随机分为4组,每组3窝仔猪。对照组(CON组)仔猪每日每头灌喂2 mL 0.1%蛋白胨溶液,植物乳杆菌组(LP组)、罗伊氏乳杆菌组(LR组)和联合添加组(PR组)仔猪每日每头分别灌喂2 mL 0.1%蛋白胨溶解的1.0×10^(10)CFU植物乳杆菌、1.0×10^(10)CFU罗伊氏乳杆菌以及1.0×10^(10)CFU植物乳杆菌+1.0×10^(10)CFU罗伊氏乳杆菌。仔猪由各自母猪哺乳,各组母猪饲喂相同的饲粮。试验期10 d。试验结束后,计算仔猪平均日增重和腹泻率。每组选取6头仔猪屠宰取样,测定肠道形态和紧密连接蛋白基因表达以及盲肠菌群。结果表明:1)与CON组相比,PR组仔猪末重和平均日增重显著提高(P<0.05),LP组、LR组和PR组腹泻率均显著降低(P<0.05)。2)与CON组相比,LP组、LR组和PR组仔猪回肠绒毛高度和绒隐比均显著提高(P<0.05)。3)与CON组相比,LP组仔猪空肠闭锁小带蛋白-1(ZO-1)和回肠封闭蛋白-1(claudin-1)mRNA相对表达量显著提高(P<0.05),LR组空肠闭合蛋白(occludin)mRNA相对表达量显著提高(P<0.05),PR组空肠occludin和claudin-1以及回肠occludin、claudin-1和ZO-1 mRNA相对表达量均显著提高(P<0.05)。4)与CON组相比,LP组、LR组和PR组仔猪盲肠菌群Ace指数和Chao1指数显著提高(P<0.05),盲肠拟杆菌属(Bacteroides)相对丰度显著降低(P<0.05),盲肠未分类普雷沃氏菌科(unclassified_Prevotellaceae)相对丰度显著提高(P<0.05);LP和PR组盲肠乳杆菌属(Lactobacillus)相对丰度显著提高(P<0.05)。由此可见,植物乳杆菌和罗伊氏乳杆菌能通过改善哺乳仔猪肠道形态,提高肠道紧密连接蛋白表达,调节肠道菌群结构,从而促进肠道健康,降低腹泻率,且两菌联合使用效果优于单一菌。This experiment was conducted to investigate the effects of Lactobacillus plantarum and Lactobacillus reuteri on growth performance,intestinal function and cecal microbiota of suckling piglets.Twelve litters(8 piglets per litter)of 1-day-old healthy“Landrace×Rongchang”suckling piglets(the parity and expected date of delivery of sows were similar)were randomly divided into 4 groups with 3 litters per group.Piglets in the control group(CON group)were fed 2 mL 0.1%peptone solution per head every day,and those in Lactobacillus plantarum group(LP group),Lactobacillus reuteri group(LR group)and combined supplementation group(PR group)were supplemented with 1.0×10^(10)CFU Lactobacillus plantarum,1.0×10^(10)CFU Lactobacillus reuteri and 1.0×10^(10)CFU Lactobacillus plantarum+1.0×10^(10)CFU Lactobacillus reuteri dissolved in 2 mL 0.1%peptone solution per head every day,respectively.Piglets were suckled by their own sows,and sows in each group were fed the same diet.The experiment lasted for 10 days.After the experiment,the average daily gain and diarrhea rate of piglets were calculated.Six piglets from each group were slaughtered and sampled to determine intestinal morphology,tight junction protein gene expression and cecal microbiota.The results showed as follows:1)compared with CON group,the final weight and average daily gain of piglets in PR group were significantly increased(P<0.05),and the diarrhea rate in LP,LR and PR groups was significantly decreased(P<0.05).2)Compared with CON group,the villus height and ratio of villus height to crypt depth in ileum of piglets in LP,LR and PR groups were significantly increased(P<0.05).3)Compared with CON group,the relative mRNA expression levels of zonula occludens-1(ZO-1)in jejunum and claudin-1 in ileum of piglets in LP group were significantly increased(P<0.05),the occludin mRNA expression level in jejunum in LR group was significantly increased(P<0.05),and the mRNA expression levels of occludin and claudin-1 in jejunum as well as occludin,claudin-1 and ZO-1 in ile

关 键 词：乳杆菌 哺乳仔猪 生长性能 肠道形态 紧密连接蛋白 肠道菌群 

分 类 号：S816.7[农业科学—饲料科学]